| 唐菖蒲愈伤组织的诱导及植株再生 |
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| 引用本文： | 刘志洋,车代弟. 唐菖蒲愈伤组织的诱导及植株再生[J]. 分子植物育种, 2004, 2(5): 695-698 |
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| 作者姓名： | 刘志洋 车代弟 |
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| 作者单位： | 东北农业大学园艺学院园林系,哈尔滨,150030;东北农业大学园艺学院园林系,哈尔滨,150030 |
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| 基金项目： | 本研究得到黑龙江省自然科学基金资助(编号C01-05). |
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| 摘 要： | 本试验以唐菖蒲无菌试管苗叶片、茎段、幼芽为外植体，探讨其愈伤组织诱导及植株再生情况。结果表明叶片愈伤组织诱导率极低。茎段愈伤多为黄色致密的非胚性愈伤，幼芽愈伤多为乳白色胚性愈伤。幼芽的愈伤组织诱导率和分化率以及分化速度也均高于茎段外植体。初步表明幼芽是建立愈伤组织再生体系的良好外植体。最佳愈伤组织诱导培养基为MS 2，4-D4．0 6BA0．5。
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| 关 键 词： | 唐菖蒲 外植体 组织培养 愈伤组织 植株再生 |
Callus Induction and Plant Regeneration of Gladiolus |
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| Liu Zhiyang Che DaidiLandscape Department,Horticulture College,Northeast Agricultural University,Harbin, Corresponding author,ccccdd@.net. Callus Induction and Plant Regeneration of Gladiolus[J]. Molecular Plant Breeding, 2004, 2(5): 695-698 |
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| Authors: | Liu Zhiyang Che DaidiLandscape Department Horticulture College Northeast Agricultural University Harbin Corresponding author ccccdd@.net |
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| Affiliation: | Liu Zhiyang Che Daidi*Landscape Department,Horticulture College,Northeast Agricultural University,Harbin,150030 * Corresponding author,ccccdd@263.net |
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| Abstract: | We used leaves, stems and young buds from tissue cultured Gladiolus plants as explants to study callus induction and plant regeneration in the experiment. The result indicated that the ratio of leaves callus induction was very low, most of stems callus were no-embryonic callus with yellow color and compact structure. Most of young buds callus were embryonic callus with white color. Young buds ratio of callus induction, differentiation and rate of differentiation were all higher than stems. We would like to suggest that young buds could be used as ideal explants for callus induction with optium medium (MS+2,4-D4.0+6BA0.5). |
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| Keywords: | Gladiolus Explants Tissue culture Callus Plant regeneration |
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