| 牦牛,黑白花牛及其远缘杂种RAPD标记的初步研究 |
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| 引用本文： | 魏亚萍,张周平.牦牛,黑白花牛及其远缘杂种RAPD标记的初步研究[J].中国牛业科学,1999,25(5):16-18. |
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| 作者姓名： | 魏亚萍 张周平 |
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| 作者单位： | 青海省畜牧兽医科学院!青海西宁810003 |
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| 摘 要： | 本文用８０个具有１０个碱基的随机引物对牦牛、黑白花牛及犏牛的基因组ＤＮＡ进行了ＰＣＲ扩增。结果表明有７个引物扩增出清晰且具多态特征的条带，其中，Ｆ１５（ＣＣＡＧＴＡＣＴＣＣ，５６４－１２５ｂｐ）可作为区分牦牛与黑白花牛的重要 的。 试品种相似性指数分析表黑白花牛与犏牛的遗传距离最小（０．５９４２）；黑白花与牦牛的遗传距离最大（０．６４５６）；而犏牛与牦牛的遗传距离居中（０．６４１０）。群体遗传多样
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| 关 键 词： | 牦牛 犏牛 黑白花牛 RAPD 遗传距离 遗传多样性 |
Preliminary studies on RAPD Markers of Yak, Holstein and Its Outbreds |
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| WEI Ya ping,ZHANG Zhou ping.Preliminary studies on RAPD Markers of Yak, Holstein and Its Outbreds[J].China Cattle Science,1999,25(5):16-18. |
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| Authors: | WEI Ya ping ZHANG Zhou ping |
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| Abstract: | PCR amplification was conducted for genome DNA of Yak, Holstein and pian niu using 80 10bp random primers. 7 primers amplified the clear polymorphic bands, and F 15 (CCAGTACTCC) could be used as an important marker of distinguishing yak and holstein. Similarity index analysis of tested breeds indicated that the smallest (0 5942), the biggest (0.6456), and the moderate (0.6410) genetic distance existed between Holstein and pianniu, Holstein and yak, and pian niu and yak. The population genetic diversity index of yak, Holstein, and pien niu was 0.386, 0.412, and 0.498 respectively. |
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| Keywords: | yak pian niu Holstein RAPD genetic distance genetic diversity |
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