诺氟沙星对两种鲟体内SOD活力影响的比较研究

将诺氟沙星按照0、20、40、60、80和100 mg/kg浓度,对小体鲟及史氏鲟连续口服给药5 d,停药2 d后对其血浆及肝脏组织中过氧化物岐化酶(SOD)活力进行测定,以期掌握不同诺氟沙星给药浓度下,两种鲟两种组织中SOD活力变化趋势,并探讨评价该药在鲟养殖过程中使用的最适剂量,以及对鱼类肝脏的损伤情况。试验结果表明,两种鲟两种组织内均含有一定量的SOD酶,且在对照组及所有给药组肝脏中酶活力均高于血浆中。不同给药浓度下,两种鲟两种组织中SOD活力变化均呈现规律的先受诱导升高,而后被抑制降低的变化趋势,且在40 mg/kg给药浓度组达到最大值。血浆中SOD活力受给药浓度影响较小,起伏较平稳,在40 mg/kg给药浓度组史氏鲟血浆中SOD值高于小体鲟,而其他组别均为小体鲟较高。而肝脏中SOD活力变化较剧烈,且在对照组、诱导最高SOD活力的40 mg/kg给药浓度组和最高给药浓度组,小体鲟均高于史氏鲟,且小体鲟肝脏中SOD在40 mg/kg给药浓度组活力值极高,形成一个尖锐的峰值。结果表明,诺氟沙星对两种鲟给药浓度为30~50 mg/kg时,既能使药效最好而又不会对肝脏产生明显的损伤。

A Comparison Study on the Effects of Norfloxacin on the Superoxide Dismutase Activities in Two Kinds of Sturgeons

Oral drug delivery of Norfloxacin was performed on the starlet (Acipenser ruthenus Linnaeus) and the Amur sturgeon (Acipenser schrencki Brandt) for 5 days according to the concentration of 0, 20, 40, 60, 80 and 100 mg/kg, respectively. The enzyme activities of superoxide dismutase (SOD) in the blood plasma and liver tissues were measured after drug withdrawal for 2 days. This study was aimed at knowing well about the SOD change trend in both two sturgeons under the different concentration of drug delivery, and analyzing the best concentration of Norfloxacin and the liver injury during the sturgeon culture. The results showed that SOD enzyme existed in both two sturgeons with a different degree and the SOD activities were higher in the livers than in the blood plasma no matter whether the drug was delivered or not. The change of SOD activities performed the same regular trend in the two kinds of tissues under the different drug delivery concentration, which increased by inducing firstly but then fell down by inhibition lately with a maximum value under the 40 mg/kg concentration. In the blood plasma, the SOD activities hardly changed through the period of drug delivery, which were higher in the starlet than in the Amur sturgeon under 40 mg/kg and lower under other kinds of drug delivery concentrations. However, the SOD activities fluctuated sharply through drug delivery in the liver tissues. Additionally, the SOD activities were higher in the starlet than in the Amur sturgeon under 0, 40,100 mg/kg. Among of them, SOD activities in the starlet were the highest under 40 mg/kg and produced a sharp peak value. This study suggested that Norfloxacin had a better effective and could not lead to liver injury when delivered with 30 to 50 mg/kg concentration.