| 利用EST-SSR标记鉴定大白菜杂交种纯度的研究 |
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| 引用本文： | 张庶,周新成,李利斌,刘立锋,李化银,宋梅芳,高建伟,高玲. 利用EST-SSR标记鉴定大白菜杂交种纯度的研究[J]. 天津农业科学, 2010, 16(6): 1-4. DOI: 10.3969/j.issn.1006-6500.2010.06.001 |
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| 作者姓名： | 张庶 周新成 李利斌 刘立锋 李化银 宋梅芳 高建伟 高玲 |
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| 作者单位： | 山东省农业科学院蔬菜研究所山东省设施蔬菜生物学重点实验室国家蔬菜改良中心山东分中心;青岛农业大学生命科学学院; |
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| 基金项目： | 国家973前期研究专项项目，山东省农业科学院创新基金，国家科技基本条件平台建设项目 |
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| 摘 要： | 利用EST-SSR分子标记技术,选用自主开发的3对EST-SSR引物,对13个大白菜品种的总DNA进行了PCR扩增。结果表明:EST-SSR标记可以体现品种内的一致性、亲本间以及品种间的多态性,具有共显性、稳定性和可重复性的特点;EST-SSR标记可以快速、准确地鉴别大白菜品种的真实性和杂交种纯度,为大白菜种子质量的高效检测提供科学依据。
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| 关 键 词： | 大白菜 种子纯度 鉴定 EST-SSR标记 |
Use of EST-SSR Markers to Identify the Seed Purity of Chinese Cabbage Cultivars |
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| ZHANG Shu,ZHOU Xin-cheng,LI Li-bin,LIU Li-feng,LI Hua-yin,SONG Mei-fang,GAO Jian-wei,GAO Ling. Use of EST-SSR Markers to Identify the Seed Purity of Chinese Cabbage Cultivars[J]. Tianjin Agricultural Sciences, 2010, 16(6): 1-4. DOI: 10.3969/j.issn.1006-6500.2010.06.001 |
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| Authors: | ZHANG Shu ZHOU Xin-cheng LI Li-bin LIU Li-feng LI Hua-yin SONG Mei-fang GAO Jian-wei GAO Ling |
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| Affiliation: | ZHANG Shu1,2,ZHOU Xin-cheng1,LI Li-bin1,LIU Li-feng1,LI Hua-yin1,SONG Mei-fang1,GAO Jian-wei1,GAO Ling2 (1.Institute of Vegetables,Shandong Academy of Agricultural Sciences,Shandong Key Laboratory of Greenhouse Vegetable Biology,Shandong Branch of Natonal Vegetable Center,Jinan,Shandong 250100,China,2.College of life Science,Qingdao Agricultural University,Qingdao,Shandong 266109,China) |
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| Abstract: | The seed purity of 13 Chinese cabbage cultivars by the PCR-base assays with the templates of total DNAs and three EST-SSR primers developed from GenBank Database were identified. The results showed that there were significant polymorphisms among different cultivars or betwwen parents. The co-dominant bands were also found among the hybrids and their parents in our experiments. Concludely, the EST-SSR markers could be used to assess genetic purity of Chinese cabbage hybrid seeds and identify different cultiv... |
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| Keywords: | Chinese cabbage (Brassica rapa L .ssp. pekinensis) seed purity identification EST-SSR markers |
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