New analogs of pochonicine,a potent β-N-acetylglucosaminidase inhibitor from fungus Pochonia suchlasporia var. suchlasporia TAMA 87 |
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Authors: | Yuhichi Mushiake Aya Tsuchida Asami Yamada Hiroshi Kanzaki Toru Okuda Teruhiko Nitoda |
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Institution: | 1.Graduate School of Environmental and Life Science, Okayama University, Okayama 700–8530, Japan;2.HyphaGenesis Inc. |
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Abstract: | Three novel analogs of pochonicine (1) were isolated from a solid fermentation culture of the fungal strain Pochonia suchlasporia var. suchlasporia TAMA 87, and their structures were elucidated as 7-deoxypochonicine (2), 6-deoxypochonicine (3), and 6,7-dideoxypochonicine (4). These analogs were found to possess the same stereochemistry as pochonicine. Comparison of β-N-acetylglucosaminidase (GlcNAcase) inhibitory activity between these analogs and pochonicine suggested that the C-6 hydroxy group of pochonicine was essential to its potent GlcNAcase inhibitory activity and that the C-7 hydroxy group also contributed to the activity, but to a lesser extent than the C-6 hydroxy group. |
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Keywords: | pochonicine β -N-acetylglucosaminidase inhibitor Pochonia suchlasporia chitinolytic enzyme fungi solid state fermentation |
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