Analysis of blood clotting with the total thrombus analysis system in healthy dogs |
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| Authors: | Tomoko Iwanaga Ryuji Fukushima Tomoka Nagasato Ikuro Maruyama Naoki Miura |
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| Affiliation: | Departments of Joint Faculty of Veterinary Medicine, Kagoshima University Veterinary Teaching Hospital, Kagoshima University, Kagoshima, Japan;Cooperative Department of Veterinary Medicine, Animal Medical Center, Tokyo University of Agriculture and Technology, Tokyo, Japan;Graduate School of Medical and Dental Science, System Biology in Thromboregulation, Kagoshima University, Kagoshima, Japan |
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| Abstract: | To date, coagulation tests are unable to reflect in vivo coagulation status in the same system, including platelet function, fibrin clot formation, and whole blood flow. The Total Thrombus Analysis System (T-TAS), which is a microfluidic assay that simulates conditions in vivo, measures whole blood flow at defined shear rates under conditions designed to assess platelet function (PL-chip) or coagulation and fibrin clot formation (AR-chip). The T-TAS records occlusion start time, occlusion time, and area under the curve. We evaluated this test in healthy control dogs. We also investigated the effect in vivo of acetylsalicylic acid (ASA), and the effect in vitro of an anticoagulation drug (dalteparin; low-molecular-weight heparin; LMWH). The CV of the AUC of both chips was good (CVs of 6.45% [PL] and 1.57% [AR]). The inhibition of platelet function by ASA was evident in the right-shift in the PL test pressure curve. The right-shift in the AR test pressure curves showed that the administration of LMWH inhibited both platelets and the coagulation cascade. The T-TAS may be useful in the evaluation of canine blood coagulation. |
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| Keywords: | acetylsalicylic acid dalteparin dogs low-molecular-weight heparin microflow-chamber system |
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