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| 硝酸银诱导的大豆子叶中大豆Kunitz胰蛋白酶抑制剂的研究 | |
| 引用本文： | 乞永艳,刘富海,Arnaud Bovy,Jan Cordewener. 硝酸银诱导的大豆子叶中大豆Kunitz胰蛋白酶抑制剂的研究[J]. 河北农业大学学报, 2007, 30(4): 19-23 |
| 作者姓名： | 乞永艳 刘富海 Arnaud Bovy Jan Cordewener |
| 作者单位： | 农业部规划设计研究院农业发展与投资研究所,北京,100026;北京中健高科科技中心,北京,100097;Plant Research International, 6700 AJ, Wageningen, the Netherlands |
| 基金项目： | 荷兰瓦赫宁根大学交叉研究与教育基金 |
| 摘 要： | 采用二维电泳技术对硝酸银诱导的大豆子叶微粒体膜蛋白进行分离,利用四极杆-飞行时间串联质谱,对仅存在于诱导样品而不存在于对照样品中的蛋白质点进行氨基酸序列测定和分析,以期研究硝酸银处理对大豆子叶微粒体蛋白质的影响。结果显示:硝酸银可以诱导大豆Kunitz胰蛋白酶抑制剂(SKTI)的产生,在诱导处理的子叶中大量存在其成熟形式,也存在其5 kDa小亚基,小亚基含有“GIGTIISSPER”和“FIAEGNPLR”氨基酸序列。该研究结果为SKTI的深入研究提供了实践依据,并为其分离纯化提供了简便途径,从而有利于对其更多性质的进一步研究。 |
| 关 键 词： | 大豆 硝酸银 诱导 Kunitz胰蛋白酶抑制剂 二维电泳 四极杆-飞行时间串联质谱 |
| 文章编号： | 1000-1573(2007)04-0019-05 |
| 修稿时间： | 2006-12-19 |
Study on the soybean Kunitz trypsin inhibitor (SKTI) induced by silver nitrate |
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| QI Yong-yan,LIU Fu-hai,Arnaud Bovy,Jan Cordewener,Froukje vander wal. Study on the soybean Kunitz trypsin inhibitor (SKTI) induced by silver nitrate[J]. Journal of Agricultural University of Hebei, 2007, 30(4): 19-23 | |
| Authors: | QI Yong-yan LIU Fu-hai Arnaud Bovy Jan Cordewener Froukje vander wal |
| Abstract: | Soybean cotyledons were induced by silver nitrate.Microsomal membrane bounded proteins were separated by two-dimensional electrophoresis(2DE).Effect on microsomal proteins of silver nitrate was studied in the work.Protein spots that existed in the induced sample but not in the control were detected and analyzed by quadrupole-time of flight/mass spectra(Q-TOF/MS/MS).The findings bindicate that soybean Kunitz trypsin inhibitor(SKTI) can be induced by silver nitrate.There was plenty of mature SKTI and less 5 kDa subunit in the induced cotyledons.The 5 kDa subunit includes two amino acid sequence "GIGTIISSPER"and"FIAEGNPLR".The results supplied practical foundation and simple separation method for the further study of SKTI. |
| Keywords: | soybean silver nitrate induce Kunitz trypsin inhibitor two-dimensional electrophoresis quadrupole-time of flight/mass spectra |
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