| 常山提取物和常山乙素对小鼠脾淋巴细胞增殖的影响 |
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| 引用本文： | 郭志廷,韦旭斌,梁剑平,王学红,尚若锋,郭文柱,郝宝成,张永红.常山提取物和常山乙素对小鼠脾淋巴细胞增殖的影响[J].兽医大学学报,2013(11):1730-1732,1757. |
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| 作者姓名： | 郭志廷 韦旭斌 梁剑平 王学红 尚若锋 郭文柱 郝宝成 张永红 |
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| 作者单位： | [1]中国农业科学院兰州畜牧与兽药研究所农业部兽用药物创制重点实验室／甘肃省新兽药工程重点实验室,甘肃兰州730050 [2]吉林大学畜牧兽医学院,吉林长春130062 [3]二连浩特出入境检验检疫局,内蒙古二连浩特011100 |
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| 基金项目： | 中央级公益性科研院所专项基金资助项目（1610322011004） |
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| 摘 要： | 为了明确常山提取物和常山乙素的免疫学活性，应用MTT法测定2种药物体外对小鼠脾T、B淋巴细胞增殖的影响。结果显示，常山碱提取物质量浓度达到200mg／L时显著抑制了小鼠脾淋巴细胞的增殖（P〈0．05）；质量浓度在10mg／L时对ConA诱导T淋巴细胞增殖有显著的促进作用（P〈0．05）；质量浓度在10～200mg／L对LPS诱导B淋巴细胞增殖有一定的促进作用（P〉0．05）。常山乙素质量浓度达到0．1mg／L时显著抑制了小鼠脾淋巴细胞的增殖（P〈0．05）；质量浓度在0．001～0．01mg／L时对ConA诱导T淋巴细胞增殖有一定的促进作用（P〉0．05），质量浓度在0．0001mg／L时具有显著的促进作用（P〈0．05）；质量浓度在0．0001～0．005mg／L对LPS诱导B淋巴细胞增殖有一定的促进作用（P〉0．05）。结果表明，常山提取物和常山乙素具有良好的免疫增强活性，为今后相关研究提供了免疫学参考。
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| 关 键 词： | 常山提取物 常山乙素 淋巴细胞 免疫 |
Effect of radix dichroa extractive and febrifugine on mouse spleen lymphocyte proliferation |
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| Authors: | GUO Zhi ting＇ WEI Xu-bin LIANG Jian-ping WANG Xue-hong SHANG Ruo-feng GUO Wen- zhu HAO Bao-chengl＇ ZHANG Yong-hong |
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| Institution: | 1. Key Laboratory of Veterinary Pharmaceutics Discovery ,Ministry of Agriculture, Key Laboratory of New Animal Drug Project ,Gansu Prov- ince ,Lanzhou Institute of husbandry and Pharmaceutical Sciences of CAAS, Lanzhou 730050, China; 2. College of Animal Science and Veterinary Medicine, Jilin University, Changchun 130062, China ; 3. Erenhot Entry-Exit Inspection and Quarantine Bureau, Erenhot, Inner Mongo- lia 011100,China) |
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| Abstract: | In order to investigate immunological activity of radix dichroa extractive and febrifugine, proliferation of mouse spleen T lymphocyte and B lymphocyte was determined by MTT assay in vitro. The results showed that proliferation of mouse spleen lymphoeytes were significantly inhib- ited by radix dichroa extractive when its concentration was up to 200 mg/L（P〈0.05） ;ConA in- duced T lymphocyte proliferation was significantly promoted by radix dichroa extractive at 10 mg/L concentration（P〈0.0S） ;LPS-induced B lymphocyte proliferation was also prometed when its concentration ranged from 10 mg/L to 200 mg/L（P〈0.05）. Proliferation of mouse spleen lymphocyte was significantly inhibited by febrifugine when its concentration reached 0.1 mg/L（P 〈0.05） ;ConA-induced T lymphocyte proliferation was prometed when its concentration ranged from 0. 001 mg/L to 0.01 mg/L（P〈0.05） and were significantly prometed at 0. 000 1 mg/L con- centration（P〈0.05） ;LPS-induced B lymphocyte proliferation was prometed by febrifugine when its concentration ranged from 0.000 1 mg/L to 0.005 mg/L（P〈0.05）. The results suggested that radix dichroa extractive and febrifugine have better immune-enhancing activity. |
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| Keywords: | radix dichroa extractive febrifugine lymphocyte immune |
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