复方鸡脾组织提取液及紫术散对鸡新城疫疫苗的增益效应

复方鸡脾组织提取液和中药紫术散可使鸡新城疫(ND)疫苗诱导的鸡血清抗体效价显著上升,维持时间延长,并能提高鸡外周血B淋巴细胞EAC花环形成率、T淋巴细胞ANAE阳性率和大颗粒淋巴细胞(LGL)的百分率.由此认为,这两种制剂对鸡ND苗诱导的体液免疫和细胞免疫都具有促进效应,可作为免疫增效剂.

Immunopotentiating Effects of the Chinese Traditional Medicine Zi Zhu San and Extract Liquor of Chicken Spleen on ND Vaccine in Chicken

The Chinese traditional medicine -Zi Zhu San(Pulvis, its major ingredients: cow placentae, Atractylis chinensis DC) was used singly or in combination with extract liquor of chicken spleen as biological response modifiers for evaluating the immunopotentiating effects on ND vaccine in chicken. The former preparation was incorporated into feeds and the latter used for diluting ND vaccine applied. The birds of both exprimental and control groups were instilled with ND vaccine through eyes and nostrils at 15 days of age, and then their humoral and cellular immune responses were investigated successively. The results showed that in experimental groups the HI antibody levels in sera rose significantly and maintained for a more prolongation. The EAC -rosette forming rate of lymphocyte and the percentage of ANAE - positive lymphocyte and large granule lymphocyte in the peripheral blood in experimental groups were higher than those of control group. It suggested that these two preparations could have enhanced both the humoral and cellular immune responses induced by ND vaccine and thus could be used as immunopotentiating agents.