诺丽叶片DNA的提取及ISSR-PCR 反应体系的建立 |
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引用本文: | 吴田,蓝增全,李青红.诺丽叶片DNA的提取及ISSR-PCR 反应体系的建立[J].安徽农业科学,2010,38(17):8859-8860,8862. |
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作者姓名: | 吴田 蓝增全 李青红 |
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作者单位: | 西南林学院园林学院,云南昆明,650224;西南林学院环境科学系,云南昆明,650224 |
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基金项目: | 国家林业局"948"项目,西南林学院园林植物与观赏园艺云南省重点学科基金项目 |
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摘 要: | 目的]提取诺丽(Morinda citrifoliaLinn.)叶片DNA,并建立ISSR-PCR反应体系。方法]以3份采自海南、4份采自美国的诺丽种质的叶片为材料,对其DNA提取和ISSR分子标记方法进行了研究。结果]采用改进的CTAB DNA微量提取法,可以得到高质量的诺丽叶片基因组DNA。用14条不同的ISSR引物对所提取的诺丽基因组DNA进行了ISSR分子标记分析,其中7条引物在诺丽DNA中可扩增出多态性产物。结论]建立了诺丽叶片基因组DNA快速、高效的提取方法和ISSR标记体系,可为ISSR分析应用于诺丽遗传研究奠定良好的基础。
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关 键 词: | 诺丽 DNA提取 ISSR-PCR反应 |
Isolation of Genomic DNA in Morinda citrifolia Linn. and Establishment of Its ISSR-PCR Reaction System |
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Institution: | WU Tian et al(College of Architecture,Southwest Forestry University,Kunming,Yunnan 650224) |
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Abstract: | Objective] The research aimed to isolate the genomic DNA in Morinda citrifolia Linn.and establish its ISSR-PCR reaction system.Method] The Morinda citrifolia Linn.leaves of 3 germplasm resources from Hainan and 4 germplasm resources from America were chose as materials,the methods of isolating DNA in Morinda citrifolia Linn.and establishment of its ISSR-PCR were studied.Result]The results showed that the good quality DNA of Morinda citrifolia Linn.leaves were gained by the modified CTAB method.The isolated DNA could be used for ISSR analysis and 7 of 14 ISSR primers revealed polymorphisms in the Morinda citrifolia Linn.leaves DNA.Conclusion]The rapid and efficient extraction method and the ISSR analysis procedure in this experiment laid a good foundation for application of ISSR in Morinda citrifolia Linn.genetic research. |
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Keywords: | Morinda citrifolia Linn DNA isolation ISSR-PCR reaction |
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