百合抗病无性系抗枯萎病的蛋白质组学分析

以百合抗病无性系和感病无性系为试验材料，开展了百合抗尖孢镰刀菌枯萎病的蛋白质组学研究，以期明确抗病无性系参与抗病反应的关键蛋白。利用蛋白质双向凝胶电泳技术，对被百合尖孢镰刀菌诱导0、24、48和72 h的抗病无性系和感病无性系进行差异蛋白分析。经质谱检测及数据库检索，鉴定了25个差异蛋白质点，其中10个鉴定为未知功能的蛋白，其余15个依据其相关功能分为4类，即防御反应相关蛋白、光合作用相关蛋白、糖类及能量代谢相关蛋白和热激蛋白。其中参与防御反应的病程相关蛋白（推定的抗病蛋白RPS2、推定的抗晚疫病同族蛋白R1C-3、过氧化物酶Q、抗坏血酸过氧化物酶和NBS-LRR类似蛋白）可能是百合抗病无性系介入抗病防御反应的关键蛋白。

Proteomics Analysis of Lily Blight Disease-resistant Clones

To elucidate the key proteins related to disease-resistance of lily induced by Fusarium oxysporum f. sp. lilii，protein two-dimensional gel electrophoresis technology was used to analyze profiles of the disease-resistant and susceptible clones after inoculation of Fusarium oxysporum f. sp. lilii for 0，24，48 and 72 h. Tweenty-five differentially expressed proteins were successfully identified，including 10 differentially expressed proteins identified as unknown function proteins. The rest of the 15 differentially expressed proteins can be categorized into 4 classes according to the function，namely the defense reaction，photosynthesis，carbohydrate and energy metabolism，and heat shock-related proteins. The disease related proteins participated in the defense reaction including presumption of disease-resistant protein RPS2，putative late blight resistant protein homolog R1C-3，peroxidase Q，ascorbate peroxidase and NBS-LRR-like protein，which may play key roles in the resistance against lily blight.