S–腺苷甲硫氨酸对黄瓜断根扦插苗生长及生理代谢的影响

以‘津研4号’黄瓜为试验材料,研究了S–腺苷甲硫氨酸(SAM)对断根扦插后黄瓜苗期根系生长、植株生长和生理代谢的影响。结果表明,断根幼苗基部用50μmol·L-1 SAM溶液浸泡5 min,能显著促进扦插苗根系的生长发育、叶绿素的积累、光合速率的增强以及提高植株对N、P、K的吸收能力,促进幼苗的生长。进一步分析表明,SAM处理增加了黄瓜下胚轴内多胺和生长素的积累及其相关基因的表达,可能是SAM促进黄瓜根系再生和幼苗生长发育的机理。

Effect of S-adenosylmethionine on Growth and Physiological Metabolism of Cucumber Cutting Seedlings

‘Jinyan 4’cucumber was used as experimental material to study the effect of exogenous S-adenosylmethionine（SAM）on root generation，seedling growth and physiological metabolism. The results showed that the root growth，chlorophyll accumulation，photosynthetic rate were significantly improved in cucumber cutting seedlings treated with 50 μmol · L-1 SAM solution for 5 min，and N，P，K contents and cucumber seedlings growth were promoted. Further investigations indicated that SAM treatment up-regulated the expression of polyamines and auxin-related genes as well as polyamine and auxin accumulation in cucumber hypocotyls，which was the possible mechanism of SAM promoting root regeneration and plant growth of cucumber cutting seedlings.