基于 SSR 标记的克氏原螯虾种质资源遗传多样性研究

选用10对克氏原螯虾微卫星引物对桐庐(TL)、太湖(TH)、湖北(HB)、江西(JX)、洪泽湖(HZH)、崇明(CM)、广西(GX)、海盐(HY)8个克氏原螯虾群体的遗传多样性状况和遗传结构进行研究。结果发现,8个克氏原螯虾群体的平均观测杂合度值为0.525 7~0.740 0,平均期望杂合度值在0.623 8~0.743 4之间,平均多态信息含量在0.420 9~0.684 0之间。其中广西(GX)群体遗传多样性最高,太湖(TH)群体遗传多样性最低。采用MEGA4.0计算各地理种群间的遗传距离,遗传距离最远的是崇明(CM)和江西(JX)群体,遗传距离最近的是湖北(HB)和太湖(TH)群体。结果显示,这8个地理种群资源遗传多样性较高。

Genetic diversity of the red swamp crayfish(Procambarus clarkii) resources based on SSR markers

Genetic diversity of 8 wild stocks of red swamp crayfish (Procambarus clarkii )from Tonglu (TL),Taihu Lake (TH),Hubei (HB),Jiangxi (JX),Hongze Lake (HZH),Chongming (CM), Guangxi (GX),and Haiyan (HY),were investigated using 10 microsatellite DNA loci.In these 8 popula-tions,the observed heterozygosity (H o ),expected heterozygosity (H e ),and polymorphism information content (PIC)of these populations ranged from 0.525 7 to 0.740 0,from 0.623 8 to 0.743 4,and from 0.420 9 to 0.684 0,respectively,indicating a high level of genetic diversity.The highest and lowest ge-netic diversity were found in the Guangxi population and Taihu population,respectively.Then,genetic distance was calculated using the MEGA 4.0 software.And the genetic distance between HB population and TH population was the smallest,while that between CM population and JX population was the lar-gest.