电热温床处理无花果硬枝扦插生根的植物激素水平分析

为探寻电热温床催根的生理和分子生物学机制,以无花果一年生枝为材料,筛选与电热温床处理促生根效果近似的吲哚丁酸(IBA)处理条件,比较插条生根过程中主要内源激素水平的变化。结果表明:500mg/L IBA浸泡插条基部与2h与28℃电热温床处理12d的促生根效果相似。插条基部组织激素水平测定显示,IBA和电热温床处理均能在处理开始后0~10d快速而显著地提高样品中吲哚丁酸(IAA)和玉米素(ZR)的浓度;与IBA处理相比,电热温床处理显著提高了早期样品中的脱落酸(ABA)浓度;不同处理和对照样品中的赤霉素(GA)含量在0~10d差别不大,后期以IBA处理的样品中的GA含量最高。研究结果显示在启动硬枝扦插生根的激素响应模式上,电热温床和IBA处理既有共同点,又存在独特性。

Change of endogenous plant hormone levels in fig hardwood cuttings during rooting process induced by electric hotbed

Hardwood cutting is one of the most important materials in vegetative propagation of fruit and forest trees.Electric hotbed treatment is a widely used method in prompting adventitious root formation of the hardwood cuttings.However,the mechanism of this process is not yet well elucidated physiologically and molecular biologically.In the present study,we recruited hotbed and IBA treatment conditions which could lead to the similar rooting results of one year old fig (Ficus carica L.) hardwood cuttings and compared the hormone levels during the rooting process.The results demonstrated:eminent and comparable rooting results were gained from treating the cuttings 12 days on hotbed and soaking the lower end of the cuttings in 500 mg/L IBA for 2 hours.Plant hormone assay showed that IAA and zeatin level in the lower end tissue of the cuttings were significantly up-regulated in hotbed and IBA treatments;samples of hotbed treated cuttings were of remarkable higher abscisic acid content than that of IBA treatment in the early stage of the rooting process;there was no significant difference on gibberellin level between the two treatments during 0-10 DAT,while in the late stage of the process samples from IBA treatment has the highest gibberellin level.Our study revealed that hotbed and IBA treatment caused many in common in the plant hormone response model in cuttings,at the same time hotbed treatment has its uniqueness.The results lay grounds for further mechanism study on hotbed treatment led rooting response of fig cuttings.