甘蓝型油菜萝卜质雄性不育恢复系的选育

为了获得甘蓝型油菜萝卜细胞质雄性不育(Cytoplasmic male sterility,CMS)高含油量双低品质恢复系,以Ougra-CMS不育胞质杂交种Oug-F1(S(Rfrf))为不育基因和恢复基因供体,高含油量双低亲本D89-44(N(rfrf)为受体,通过杂交、多代同交以及最后自交对恢复基因进行了定向转育...

Breeding of Restorer of Radish Cytoplasmic Male Sterile Lines in Rapeseed (Brassica napus L.)

In order to obtain restorers of radish cytoplasmic male sterile lines in Brassica napus with high oil content and double-low quality traits, Oug-F1 of B. napus L. was used as donor, which carries Ougra-CMS cytoplasm and restorer gene (its genotype was S(Rfrf)), the parental line D89-44 as receptor, which has normal cytoplasm of rapeseed, with high oil content, lower erucic acid, low glucosinolate contents and excellent economical characters (its genotype is N(rfrf)), directional transferring method was conducted to transfer Rf gene into receptor line D89-44 through hybridization between Oug-F1 (donor) and D89-44 line (receiver) followed by multiple backcrossing. The molecular marker assisted selection (AMS) was performed using specefic prime P2 for sterility gene and P5 for restorer gene in every backcrossing and inbred generation, selected plants as female parent for backcrossing with receptor line D89-44 were sprayed with 7 mg/L of chemical hybrid agent (CHA) SX-1 at later mononuclear pollen stage with 1~2 mm long flower bud and 5 mg/L at near blooming, respectively. The results showed that application of CHA SX-1 induced complete male sterility of selected plant and improved hybridization efficiency. Selected restorer D89-44-Rf of RCMS lines have similar agronomic traits and quality traits with D89-44. Therefore, the established procedure can be applied in high oil content and double-low quality breeding and genetic improvement of rapeseed.