Plant regeneration from mesophyll protoplast of indica rice Qiugui＇ai 11 （Oryza sativa L.）

In the recent decade, plant regeneration from protoplast has been obtained through embryogenic cell suspension cultures of rice. However, not only the establishment of embryogenic call suspension cultures of rice was difficult, but also the protoplasts became less and less regenerable and the genetic change was gradu ally accumulated during the prolonged culture.Since 1976 (Deka.), extensive efforts have been made to induce sustained division and regenerate plants from rnesophyll protoplasts of rice, but not successful.