基于环介导等温扩增技术检测东北地区大豆主要品种(系)种子携带的病原菌

为了解东北地区大豆种子携带病原菌的情况,选取了该地区52个大豆主要品种(系),采用环介导等温扩增技术(loop-mediated isothermal amplification,LAMP)快速检测其种子携带胶孢炭疽菌、平头炭疽菌、冬青丽赤壳菌、亚细亚镰孢菌、黄色镰孢菌、木贼镰孢菌、禾谷镰孢菌、层出镰孢菌、尖镰孢菌、茄腐镰孢菌、轮枝镰孢菌、接骨木镰孢菌、大豆炭腐病菌、大豆拟茎点种腐病菌、大豆疫霉菌和立枯丝核菌16种大豆主要病原菌的状况。结果表明:在其中38个品种(系)的种子样本中累计检测出上述8种病原菌,检出率由高到低依次为:大豆拟茎点种腐病菌、立枯丝核菌、木贼镰孢菌、亚细亚镰孢菌、禾谷镰孢菌、层出镰孢菌、尖镰孢菌和平头炭疽菌。不同大豆品种(系)种子带菌的种类及数量存在较大差异,其中品种绥12-18和29182被检出的病原菌多达4种。本研究对了解东北地区大豆种子携带病原菌的状况有参考价值,并为大豆种子带菌检测提供了新的方法。

Detection of Soybean Seed-borne Pathogens in Northeast China Using Loop-mediated Isothermal Amplification Assays

In order to know the situation of soybean seed-borne pathogens in Northeast China,the seeds of 52 major soybean cultivars(lines) collected from this area were analyzed,using loop-mediated isothermal amplification(LAMP) assays for specific detection of 16 known soybean pathogen species,including Colletotrichum gloeosporioide,C.truncatum,Calonectria ilicicola,Fusarium asiaticum,F.culmorum,F.equiseti,F.graminearum,F.oxysporum,F.proliferatum,F.solani,F.sambucinum,F.verticilliodes,Macrophomina phaseolina,Phomapsis longicolla,Phytophthora sojae,and Rhizoctonia solani.The result showed that there were 38 soybean cultivars(lines) carrying a total of 8 species of seed-borne pathogens,and the most frequent species was Phomapsis longicolla,followed by Rhizoctonia solani,Fusarium equiseti,F.asiaticum,F.graminearum,F.proliferatum,F.oxysporum and Colletotrichum truncatum.The number of detected species in each cultivar of seeds were between 1 and 4.In summary,a simple,rapid,and sensitive method for detecting soybean seed-borne pathogens was developed and successfully used to reveal the situation of soybean seed-borne pathogens in Northeast China.