一种生物刺激素对大豆根际土壤微生物群落的影响

为研究生物刺激素(二苯基脲磺酸钙)对大豆根际土壤微生物群落的影响,采用中华费氏根瘤菌或不同浓度的生物刺激素对汾豆78进行浸种,田间条件下播种后在大豆花期时采集大豆根际土壤,并通过对比改良SDS法、TSAI法、生工试剂盒法和Mobile试剂盒法,选取效果最佳的Mobile试剂盒法进行土壤细菌DNA提取,对目的片段16S V3区进行特异性扩增,扩增产物用于后续高通量测序分析。结果表明:5个试验组与CK组相比,试验组Shannon数值下降。处理S2、S3、S4和S5与CK相比,变形菌门、放线菌门和拟杆菌门相对含量增加;酸杆菌门和硬壁菌门相对含量减少。试验还发现在属的水平上,一些对大豆生长有益和促进固氮的功能菌种有增加趋势。生物刺激素使大豆根际土壤微生物多样性下降;可以促进根瘤菌的生长,增加根际土壤微生物中根瘤菌的含量;同时生物刺激素还可以促进根际土壤微生物中功能菌属的数量,不仅促进大豆固氮,还促进大豆生长发育。

Effect of A Kind of Biological Stimulant on Microbial Community in Rhizosphere Soil of Soybean

In this paper,field experiments were carried out to study the influence of biostimulant (Diphenylurea Sulfonic Calcium),developed by Shanxi University to the rhizosphere soil microbial community.Test set up five experimental groups,group S1 to S5,and one blank control group CK,then spray Chinese fisher Rhizobium or different concentrations of biostimulant to soybean seeds.Collected soybean rhizosphere soil during the flowering period,and compared the modified SDS method,TSAI method,Sangon magnetic bead kit and Mobile kit method,and selected the best Mobile Kit Method for soil bacterial DNA extraction.Amplified the fragment of 16S V3 region specifically,amplification products used for subsequent highthroughput sequencing analysis.The result showed that compared with the CK group,the Shannon values of the 5 experimental groups decreased.Compared with CK,S2,S3,S4 and S5 were found that the relative content of Proteobacteria,Actinomyces and Bacteroides were increased in the main phylum,and the relative contents of the phylum and the hard wall were reduced.At the genus level,it was also found that there was an increasing trend of functional bacteria in soybean growth and nitrogen fixation.Biostimulins made the diversity of soybean rhizosphere soil microorganism decreased,and could promote the growth of rhizobia,increase the content of rhizobia in rhizosphere soil microorganisms,at the same time,it could promote the number of functional bacteria in rhizosphere soil microbes,not only promote the soybean nitrogen fixation,but also promote the growth of soybean.