大豆抗腐霉根腐病的生理差异研究

腐霉根腐病是大豆主要的土传病害,为揭示抗感病大豆品种的生理差异,对不同抗性品种在腐霉病原菌侵染下的大豆下胚轴中丙二醛、苯丙氨酸解氨酶(PAL)、过氧化物酶(POD)、可溶性糖、脯氨酸含量的变化进行测定。结果表明:腐霉菌侵染后,抗病大豆品种下胚轴的丙二醛含量增加不十分明显;可溶性糖含量都是正向增加,苯丙氨酸解氨酶活性,过氧化物酶活性,脯氨酸含量在不同时段都有所增加且抗病品种明显高于感病品种。而感病品种在腐霉菌侵染条件下,大豆下胚轴中丙二醛含量累积较高,可溶性糖在某一时段内有所减少,苯丙氨酸解氨酶活性、过氧化物酶活性,脯氨酸含量增加幅度小于抗病品种,表明抗病品种具有较强的生理生化调节能力。

Physiological Differences in Soybean Resistance to Pythium Root Rot

Pythium root rot is an important soil-born diseases,revealing the physiological differences of susceptible variety has important significance in resistant varieties breeding.Using physiological techniques to detected MDA,solutions of phenylalanine ammonia-lyase (PAL),peroxidase (POD),the change of soluble sugar and proline content in different resistance cultivars under Pythium infection in soybean hypocotyls.Research results showed that after Pythium infection,in resistant cultivars' hypocotyl and malondialdehyde content were not increased obviously in resistant cultivar,and soluble sugar contents were increased.Solution of phenylalanine ammonialyase activity,peroxidase activity,proline contents increased in different phase and significantly higher than these in the susceptible varieties.Susceptible variety after Pythium ultimum infested conditions,malondialdehyde content were high and soluble sugars would reduce in a given period.Solution of phenylalanine ammonialyase activity,peroxidase activity,and proline content were increased,but less than that in resistant varieties,indicated that resistant varieties had strong physiological and biochemical regulation.