不同时期喷施APK-9对大豆受酸雨胁迫的缓解效应

为探讨叶面喷施新型酸雨缓解剂APK-9对酸雨胁迫大豆毒害效应的缓解效果,以徐豆18为供试材料,研究了不同生育时期喷施APK-9处理对pH3.5酸雨胁迫下大豆生长的影响。结果表明:pH3.5酸雨自开花期开始显著抑制了大豆植株的生长,进而降低了产量。开花期和结荚期喷施APK-9可明显缓解大豆的酸雨胁迫,氮积累量、磷积累量、叶片色素含量、抗氧化酶活性等显著高于酸雨胁迫的大豆,丙二醛(MDA)含量显著低于酸雨处理大豆。然而,苗期和鼓粒期喷施APK-9,缓解效应下降,叶绿素含量、生物量、产量等下降,而MDA含量上升。可见在适宜时期喷施APK-9对酸雨胁迫下的大豆植株具有一定的缓解效应,而过早或过晚喷施效果较小,在试验中以开花期喷施的效果最佳。

Alleviative Effects of Spraying Plant Growth Regulator APK-9 on Acid Rain Stressed Soybean Plants at Different Growth Stages

Acid rain is considered to be the top ten environment concerns in the world.In a field experiment,soybean (Glycine max L.) cultivar Xuzhou 18 was subjected to simulated acid rain (AR,pH3.5),followed by foliar application of APK-9 consisting of ascorbic acid (AsA) and polyaspartic acid.The alleviative effect of APK-9 on AR stress in soybean plants was then investigated.The results showed that AR obviously depressed growth after flowering stage and finally reduced soybean yield.Chlorophyll content,nitrogen accumulation,phosphorus accumulation,AsA content,and antioxidative enzyme activities in AR stressed seedlings were enhanced,while malonyldialdehyde (MDA) content was repressed by application of APK-9 in flowering stage and podding stage.However,chlorophyll content in leaf,biomass and yield reduced while MDA content increased when APK-9 was sprayed in seedlings stage and seed-filling stage.Thus,AR stress on soybean plants was effectively alleviated by foliar application of APK-9 in flowering stage and podding stage and the alleviation effect could be depressed by earlier or later application of APK-9.Here,spraying APK-9 in flowering stage had the greatest effect on AR stressed plants.