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花生根瘤愈伤组织的诱导和分化
引用本文:曹燕珍,李阜棣.花生根瘤愈伤组织的诱导和分化[J].华中农业大学学报,1990,9(1):47-50,T001.
作者姓名:曹燕珍  李阜棣
作者单位:华中农业大学生物固氮研究室,武汉430070
摘    要:生长健壮的花生根瘤在CLS培养基上诱导出愈伤组织,形成愈伤组织的频率达到26.5-46.6%。瘤愈伤组织能多次继代,生长良好,在分化培养基D-57和D-59上已分化形成了根,其生根频率分别为56%和78%。显微观察表明,愈伤组织分化的具有正常根的解剖结构。

关 键 词:花生根瘤  愈伤组织  诱导  分化

THE IHDUCTION OF CALLUS FROM PEANUT NODULE AND ITS DIFFERENTIATION
Cao Yanzhen,Li Fudi.THE IHDUCTION OF CALLUS FROM PEANUT NODULE AND ITS DIFFERENTIATION[J].Journal of Huazhong Agricultural University,1990,9(1):47-50,T001.
Authors:Cao Yanzhen  Li Fudi
Abstract:Nodule pieces of peanut were used to induce callus culture. The frequencies ofcallus formation from explant were 26.5-56.6% on CLS medium. Calli from peanutnodule have survived for many transfers. Upon induction the calli differentiated intorootlets on media D-57 and D-59, with frequencies of 56% and 78% respectively. Itwas shown by microscopy that the toot1et from callus revealed anatomical character ofnormal root.
Keywords:peanut nodule  callus culture
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