粮食中DON-免疫亲和层析净化高效液相色谱法的回收率方法比较

用免疫亲和层析净化高效液相色谱法测定粮食中的脱氧雪腐镰刀菌烯醇,且与GB/T 23503-2009《食品中脱氧雪腐镰刀菌烯醇的测定免疫亲和层析净化高效液相色谱法》、Romer labs实验室推荐方法、实验室优化方法进行比较。本实验方法在前两种方法的基础上优化后,在0.5mg/kg~5mg/kg的加标水平下,回收率在90.3%~100.2%,RSD在1.2%~2.0%之间,最低检出限则为44μg/kg,定量限为146μg/kg,能够很好的满足实验室分析要求。

COMPARISON OF DON RECOVERY RATE DETERMINATION BY HPLC COMBINED WITH IMMUNOAFFINITY COLUMNS

In this experiment,the HPLC combined with immunoaffinity columns had been used to detect DON,with contrast to GB/T 23503-2009,the Romer labs instructions method,and the optimized laboratory method.The optimized laboratory method improved the other methods.At 0.5 mg/kg-5 mg/kg of standard addition,the recovery rate was from 90.3％ to 100.2％,and RSD is between 1.2％ to 2.0％.Determination limit is 44 μg/kg,quantitative limit of 146 μg/kg,it can be suitfor the requirements of laboratory analysis.