链霉菌抗生物素蛋白—过氧化物酶连结法定位肝脏中脱氧雪腐镰刀菌烯醇的研究

脱氧雪腐镰刀菌烯醇(deoxynivalenol,DON),又名呕吐毒素,是目前全球谷物污染最严重的真菌毒素之一。在所有的易感动物中,以猪最为敏感。本研究选用10头健康阉公猪,随机分为2组,染毒组和对照组,染毒组经乳静脉注射DON,注射剂量为75μg/kg体重,对照组经乳静脉注射生理盐水。注射完成后开始计时30 min后放血宰杀,迅速取肝脏,采用链霉菌抗生物素蛋白—过氧化物酶连接结(streptavidin-perosidase,SP)法定位肝组织中的DON。SP法定位结果显示,在染毒组肝门管区小叶间静脉的血管壁有DON的分布。本研究采用SP法定位DON在猪体内消化器官组织的分布情况,为毒素的残留研究提供依据。

The Research of Locating DON in Liver with Streptavidin-perrosidase Method

Deoxynivalenol (DON) contamination of grains is a worldwide phenomenon. Pig is the most susceptible animal to DON. In this research, 10 healthy clean pigs were divided into 2 groups randomly. One group was given DON from breast vein with a dose of 75 μg/kg b.w.,while the other group using sodium chloride. 30 min after the intravenous(IV) administration, pigs were killed and liver tissue samples were taken out quickly. Streptavidin-perrosidase(SP) method was used to detect the DON in liver. The positioning study of SP method showed that DON could be seen in liver’s portal area(interlobular veins). This research studied the distribution of deoxynivalenol in organs and tissues of pig, offering the source for the research of residue of toxin.