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| 春甘蓝花芽分化至抽薹过程中生理生化指标的变化 | |
| 引用本文： | 杨小明,李成琼,宋洪元,任雪松,司军. 春甘蓝花芽分化至抽薹过程中生理生化指标的变化[J]. 中国蔬菜, 2009, 1(24): 19-23 |
| 作者姓名： | 杨小明 李成琼 宋洪元 任雪松 司军 |
| 作者单位： | 西南大学园艺园林学院，重庆市蔬菜学重点实验室，重庆 400716 |
| 基金项目： | 重庆市科技攻关项目，国家科技支撑计划，国家科技支撑计划 |
| 摘 要： | 以两个抽薹性不同的春甘蓝材料2006042和春丰为试材,利用石蜡切片法观察甘蓝花芽分化发育的过程,并对不同阶段发育过程中生理生化指标进行分析。结果表明:易抽薹材料2006042花芽分化进程明显早于耐抽薹品种春丰,早8 d左右;2006042的可溶性蛋白电泳分析显示在抽薹期出现了3条表达明显增强的特异蛋白,分子表达量分别为48.4、18.8、14.5 kD，同时84.0 kD条带消失，17.4 kD条带减弱，说明这几种蛋白质与抽薹密切相关；易抽薹材料2006042的可溶性蛋白质和蔗糖含量始终比耐抽薹材料春丰高。 |
| 关 键 词： | 春甘蓝 花芽分化 抽薹 生理生化指标 |
| 收稿时间： | 2009-06-14; |
Changes of Physiological and Biochemical Indexes from Budding to Bolting in Spring Cabbage |
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| YANG Xiao-ming,LI Cheng-qiong,SONG Hong-yuan,REN Xue-song,SI Jun. Changes of Physiological and Biochemical Indexes from Budding to Bolting in Spring Cabbage[J]. China Vegetables, 2009, 1(24): 19-23 | |
| Authors: | YANG Xiao-ming LI Cheng-qiong SONG Hong-yuan REN Xue-song SI Jun |
| Affiliation: | College of Horticulture and Landscape Architecture,Southwest University,Chongqing Key Laboratory of Olericulture,Chongqing 400716,China |
| Abstract: | Taking 2 different spring cabbage(Brassica oleracea L.)varieties‘2006042’and‘Chunfeng’as materials,the paper observes their development process of flower bud differentiation by petrolin section cutting method and analyzes the changes in physiological and biochemical indexes during different development stages.The results show that the budding process of bolting susceptible cabbage‘2006042’is about 8 days earlier than that of the bolting tolerant cabbage‘Chunfeng’.The analysis of soluble protein in‘2006042’with SDS-PAGE finds three special protein bands(48.4 kD,18.8 kD,14.5 kD)are expressively re-enforced,and at the same time the band(84.0 kD)is disappeared and the band(17.4 kD)is weakened.This indicates that the above mentioned proteins have very close relation with bolting.The contents of soluble protein content and sucrose in‘2006042’are higher than that in‘Chunfeng’. |
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