| 北冰洋产淀粉酶海洋细菌的筛选与鉴定及其产酶条件的优化 |
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| 引用本文： | 陈吉刚,张蓉蓉,杨季芳,毛芝娟.北冰洋产淀粉酶海洋细菌的筛选与鉴定及其产酶条件的优化[J].安徽农业科学,2011,39(1):53-55,65. |
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| 作者姓名： | 陈吉刚 张蓉蓉 杨季芳 毛芝娟 |
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| 作者单位： | 浙江万里学院生物与环境学院,浙江宁波,315100;浙江万里学院生物与环境学院,浙江宁波,315100;浙江万里学院生物与环境学院,浙江宁波,315100;浙江万里学院生物与环境学院,浙江宁波,315100 |
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| 基金项目： | 国家科技部国际合作重点项目(2007DFA21300); 宁波市重点实验室开放基金项目(2007A22007); 浙江省新苗人才计划(2008R40G2210031); 浙江省教育厅项目(20060190) |
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| 摘 要： | 目的]筛选与鉴定北冰洋产淀粉酶海洋细菌,并对筛选出的细菌进行产酶条件优化。方法]从156株北冰洋海洋细菌中筛选出淀粉酶高产菌株Arc B84A,并对其进行了菌株形态学鉴定、16S rRNA分子鉴定以及产酶条件优化。结果]菌株Arc B84A属于交替假单胞菌属。菌株Arc B84A的最佳产酶条件为：培养基起始pH值为7.0～8.0;最佳碳源为0.5%葡萄糖;最佳氮源为1.0%蛋白胨;Tri-tonX-100、Tween-20、Tween-80等表面活性剂可以提高菌株淀粉酶活性,其中1.0%Tween-80的效果最为明显。结论]该研究为海洋细菌淀粉酶的生产与应用提供了理论依据。
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| 关 键 词： | 北冰洋 淀粉酶 交替假单胞菌 |
Screening and Identification of Amylase Producing Strain from Arctic Sea |
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| CHEN Ji-gang et al.Screening and Identification of Amylase Producing Strain from Arctic Sea[J].Journal of Anhui Agricultural Sciences,2011,39(1):53-55,65. |
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| Authors: | CHEN Ji-gang |
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| Institution: | CHEN Ji-gang et al (College of Biological and Environmental Engineering,Zhejiang Wanli University,Ningbo,Zhejiang 315100) |
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| Abstract: | Objective] The aim was to investigate the screening and identification of amylase-producing marine bacteria from Arctic sea and optimization of the amylase producing conditions. Method] A high-yield strain for producing amylase named Arc B84A was isolated from a total of 156 marine bacteria from Arctic sea. Then,the morphological identification of the strain,molecular identification of 16S rRNA and optimization of fermentation conditions were conducted. Result] Arc B84A strain is a member of Pseudoaltero... |
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| Keywords: | Arctic sea Amylase Pseudoalteromonas |
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