激素IAA和ABA对小球藻（Chlorella sp.）生长和油脂积累的影响

从北部湾地区淡水水域分离得到一株高含油量小球藻藻株，将其培养在高碳培养基TAP上，研究不同浓度IAA和ABA对其生物量和油脂积累的影响。结果表明：添加10μmol/L IAA对藻株的生长有明显的促进作用，其最大生物量比对照组增加了58.24%，但其油脂含量却比对照组降低了34.71%；而当增加IAA浓度为20μmol/L时，对藻株的生长表现出明显的抑制作用，其最大生物量比对照组减少了53.60%，但其油脂含量与对照组相比显著增加，增加了95.46%。添加2μmol/L ABA对藻株生长的抑制作用极其明显，其生物量比对照组降低了57.52%，但其油脂含量与对照组相比显著提高，提高了121.49%。同时添加IAA（20μmol/L）和ABA（2μmol/L）的实验藻种的蛋白质和总糖的含量较对照组有所降低，此外也使叶绿素含量和光合效率降低。  

Effects of indole-3-acetic acid and abscisic acid on growth and lipid accumulation of Chlorella sp.

 A high oil microalgae strain collected from fresh waters of Beibu Bay area was indentified as Chlorella sp. and cultured in high-carbon medium TAP. Effects of IAA and ABA on its biomass and oil accumulation were studied. The results showed that 10μmol/L IAA promoted the growth of algae strains significantly. The max biomass increased by 58.24% compared to control while oil content decreased by 34.71%. In contrast, 20μmol/L IAA clearly inhibited the growth of algae strains and the decrease in max biomass was 53.60%. However, oil content increased by 95.46%. The inhibition of ABA on the growth of algae strains was evident. There was a 57.52% decrease in its biomass compared to control group and a 121.49% increase in its oil content. The protein, total sugar content, chlorophyll content and photosynthetic efficiency of the algae were decreased compared to control when IAA（20μmol/L）and ABA （2μmol/L）were added to the medium.

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