拮抗油菜菌核病菌的链霉菌分离筛选与鉴定

从甘肃省河西走廊改良后的盐碱地分离链霉菌用于油菜菌核病菌的拮抗研究，并对拮抗菌进行鉴定。分离得到10株不同菌落形态的链霉菌，其中有2菌株对油菜菌核病有拮抗作用，编号Ⅲ22-3-3和Ⅲ22-3-12。与油菜菌核病进行对峙培养结果发现，Ⅲ22-3-3和Ⅲ22-3-12抑菌圈直径分别为1.2和0.9cm；离体叶菌丝接种试验表明，Ⅲ22-3-3和Ⅲ22-3-12对油菜菌核病菌的防治效果分别达63.5%和49.1%；Ⅲ22-3-12能在油菜菌核上定殖并寄生分解菌核，同对照相比菌核萌发率下降18.2%；通过形态培养特征、生理生化特征和16S rDNA鉴定表明，Ⅲ22-3-3为产水链霉菌(Streptomyces hydrogenans)，Ⅲ22-3-12为球孢链霉菌(Streptomyces globisporus)。[

Identification of Streptomyces antagonizing oilseed rape pathogen Sclerotinia sclerotiorum

Abstract: Different Streptomyces strains were isolated from saline-alkali soils of Hexi Corridor in Gansu Province. Among them, two strains named Ⅲ22-3-3 and Ⅲ22-3-12 were found to be antagonists inhibiting Sclerotinia sclerotiorum isolated from oilseed rape. Detached leaf inoculation tests showed that the two strains had preventing effects of 63.5% and 49.1% respectively. Moreover, Ⅲ22-3-12 could colonize on the sclerotia and inhibit the germination of the pathogen. Compared to physiological saline (control), Ⅲ22-3-12 decreased 18.2% of the sclerotia germination rate. Morphological and physiological-biochemical characteristics as well as phylogenetic analysis based on 16S rRNA gene sequence supported strain Ⅲ22-3-3 to be Streptomyces hydogenans, and strain Ⅲ22-3-12 to be Streptomyces globisporus.

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