| 鹅副粘病毒LS-1株的分离鉴定及F基因分析 |
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| 引用本文： | 张伟,刁有祥,徐福亮,李宏梅,马艳芳,孙宁.鹅副粘病毒LS-1株的分离鉴定及F基因分析[J].西南农业大学学报,2011,33(4). |
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| 作者姓名： | 张伟 刁有祥 徐福亮 李宏梅 马艳芳 孙宁 |
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| 作者单位： | 山东农业大学动物科技学院;青岛康地恩药业有限公司; |
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| 基金项目： | 国家现代农业产业技术体系建设专项基金资助项目(nycytx-45-11); 国家公益性行业(农业)科研专项基金资助项目(201003012); 山东省科技攻关资助项目(2007GG30009001) |
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| 摘 要： | 从山东省梁山县疑似鹅副粘病毒感染的病死鹅中分离到1株LS-1病毒株,经血凝和血凝抑制试验,证实该毒株为禽1型副粘病毒.毒力测定结果表明,该病毒对鸡胚平均致死时间(MDT)为39.5 h;1日龄无特定病原体(SPF)鸡脑内接种分离病毒的致病指数(ICPI)为1.76;6周龄SPF鸡静脉接种致病指数(IVPI)为2.42.序列测定结果表明LS-1株F基因核苷酸长度为1 653 bp,编码为551个氨基酸,有6个糖基化位点,其裂解位点的氨基酸序列为112-Arg-Arg-Gln-Arg-Arg-Phe-117,与已公布的强毒株裂解位点的氨基酸序列相符.同源性分析表明,该毒株F基因与目前已公布的鹅副粘病毒F基因核苷酸同源性在87.0%~98.4%之间,推导的氨基酸序列同源性在87.0%~98.5%之间.
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| 关 键 词： | 鹅副粘病毒 F基因 RT-PCR 序列分析 |
Isolation and Identification of GPMV Strain LS-1 and Sequence Analysis of Its Fusion Protein Gene |
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| ZHANG Wei,DIAO You-xiang,XU Fu-liang,LI Hong-mei,MA Yan-fang,SUN Ning.School of Animal Science , Technology,Sh,on Agricultural University,Taian Sh,ong ,China,.Qingdao Kang Di En Medicine Limited Company,Qingdao Sh,ong.Isolation and Identification of GPMV Strain LS-1 and Sequence Analysis of Its Fusion Protein Gene[J].Journal of Southwest Agricultural University,2011,33(4). |
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| Authors: | ZHANG Wei DIAO You-xiang XU Fu-liang LI Hong-mei MA Yan-fang SUN NingSchool of Animal Science Technology Sh on Agricultural University Taian Sh ong China Qingdao Kang Di En Medicine Limited Company Qingdao Sh ong |
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| Institution: | ZHANG Wei1,2,DIAO You-xiang1,XU Fu-liang2,LI Hong-mei1,MA Yan-fang1,SUN Ning11.School of Animal Science and Technology,Shandon Agricultural University,Taian Shandong 271018,China,2.Qingdao Kang Di En Medicine Limited Company,Qingdao Shandong 266111 |
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| Abstract: | A strain of virus named LS-1 was isolated from a dead goose characterized similar to Newcastle disease.HA and HI tests showed that the strain was a paramyxovirus type 1.Virulance teats indicated that its MDT,ICPI and IVPI were 39.5 h,1.76 and 2.42,respectively.According to the sequence of the fusion genes published in GenBank,two pairs of primers were designed for amplifying the F protein gene in RT-PCR.The fusion protein gene of the LS-1 strain was amplified.The sequence of F protein gene of LS-1 strain wa... |
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| Keywords: | goose paramyxovirus virus(GPMV) fusion protein gene RT-PCR sequence analysis |
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