猪IFIT1基因的克隆、生物信息学和组织表达分析

采用cDNA末端快速扩增(RACE)方法,对荣昌猪IFIT1基因进行克隆测序,利用生物信息学方法分析其结够和功能,并用Real-ti me PCR方法分析初生个体和1月龄个体11个不同组织(肺、心、肾、舌头、膀胱、卵巢、脾、肝、肌肉、胃和小肠)的表达情况.结果表明:IFIT1基因cDNA序列为1971 bp(GenBank登录号:HQ679904),包含5 UTR48 bp,全部CDS序列1437 bp和3 UTR487 bp,编码478个氨基酸,相对分子质量为55 232.9×103,等电点为6.18.其二级结构以α-螺旋和无归卷曲为主,三级结构具有典型的三角四肽和螺旋转角螺旋重复结构.荧光定量结果显示IFIT1表达量在初生个体和1月龄个体各组织间均存在着显著性差异(p<0.01),初生个体在脾脏中的表达量高于其它各组织(p<0.01),1月龄个体在肌肉中的表达量高于其它组织(p<0.01).

Cloning of IFIT1 Gene and Its Bioinformatics and Tissue Expression Analysis in Swine

The cDNA of IFIT1 gene was cloned with RACE(rapid amplification of cDNA end)and its structures and functions were analyzed by bioinformatics.The mRNA distribution of the porcine IFIT1 was analyzed in 11 tissues(lung,heart,kidney,tongue,bladder,ovary,spleen,liver,skeletal muscle,stomach and small intestine) of newly-born and 1-month-old piglets with RT-PCR.The result showed that the sequence of IFIT1 was 1 971 bp(GenBank ID: HQ679904) in length,containing 48 bp 5' UTR and coding region(CDS,1 437 bp) and 487 ...