| 甘薯叶多酚氧化酶的分离纯化和部分性质研究 |
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| 引用本文： | 梁建荣,黄洁,苏茉,高亚朋,唐云明. 甘薯叶多酚氧化酶的分离纯化和部分性质研究[J]. 西南大学学报(自然科学版), 2011, 33(2) |
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| 作者姓名： | 梁建荣 黄洁 苏茉 高亚朋 唐云明 |
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| 作者单位： | 西南大学生命科学学院;重庆市甘薯工程研究中心;三峡库区生态环境教育部重点实验室; |
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| 基金项目： | 重庆市科委资助项目(CSCT,2004AC1012) |
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| 摘 要： | ![]()
经硫酸铵分级沉淀、DEAE-Sepharose离子交换层析、Superdex-200凝胶过滤层析,从甘薯叶中得到多酚氧化酶(PPO)电泳纯制品.酶比活力为50 075.43 U/mg,纯化倍数为597.99,回收率为0.48%,相对分子质量约为6.44×104,最适pH值为6.5,最适温度为35℃,且在25~55℃内较稳定.有机溶剂甲醇、乙醇、异丙醇对多酚氧化酶均有一定抑制作用,其抑制作用由强到弱顺序为异丙醇、乙醇、甲醇.低浓度的抗坏血酸和亚硫酸钠对多酚氧化酶有强烈的抑制作用,柠檬酸次之,EDTA-2Na的抑制效果较弱.
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| 关 键 词： | 甘薯叶 多酚氧化酶 分离纯化 性质 |
Isolation, Purification and Enzymological Characterization of Polyphenol Oxidase from Sweet Potato Leaves |
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| LIANG Jian-rong,HUANG Jie,SU Mo,GAO Ya-peng,TANG Yun-ming.School of Life Science,Southwest University,Chongqing ,China,.Chongqing Sweetpotato Research Center,.Key Laboratory of Eco-environments in Three Gorges Reservoir Region Ministry of Education. Isolation, Purification and Enzymological Characterization of Polyphenol Oxidase from Sweet Potato Leaves[J]. Journal of southwest university (Natural science edition), 2011, 33(2) |
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| Authors: | LIANG Jian-rong HUANG Jie SU Mo GAO Ya-peng TANG Yun-ming.School of Life Science Southwest University Chongqing China .Chongqing Sweetpotato Research Center .Key Laboratory of Eco-environments in Three Gorges Reservoir Region Ministry of Education |
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| Affiliation: | LIANG Jian-rong,HUANG Jie,SU Mo,GAO Ya-peng,TANG Yun-ming1.School of Life Science,Southwest University,Chongqing 400715,China,2.Chongqing Sweetpotato Research Center,3.Key Laboratory of Eco-environments in Three Gorges Reservoir Region Ministry of Education |
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| Abstract: | ![]()
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| Keywords: | sweet potato leaf polyphenol oxidase isolation and purification property |
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