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相思根瘤菌和解磷菌培养基优化及解磷能力研究
引用本文:尚军红,康丽华,罗玉萍,江业根,马海宾.相思根瘤菌和解磷菌培养基优化及解磷能力研究[J].林业科学研究,2005,18(2):177-182.
作者姓名:尚军红  康丽华  罗玉萍  江业根  马海宾
作者单位:1. 中国林业科学研究院热带林业研究所,广东广州,510520
2. 南昌大学,江西南昌,330047
基金项目:国家十五攻关项目"南方主要速生阔叶树种新品种选育及培育技术"(2002BA515B02)和国家863计划"新型多功能林用微生物肥料关键技术研究"(2003AA2411533)部分内容
摘    要:研究绘制了相思根瘤菌MZ菌株和解磷菌217菌株在4种培养基中的OD值生长曲线,结果表明:它们都能在BSE培养基中较好的生长;对MZ根瘤菌和217解磷菌的常规培养基进行优化,获得了它们的优化培养基,进一步绘制的生长曲线证明了优化培养基更利于菌株的生长,且活菌数更多。217解磷能力的研究也表明:菌体数量与pH值、解磷强度之间有着密切的关系。

关 键 词:相思  根瘤菌  解磷细菌  培养基优化  解磷能力
文章编号:1001-1498(2005)02-0177-06

Medium Optimization of Acacia Rhizobia and Phosphobacteria and the Study of Phosphate-dissolving Ability
SHANG Jun-hong,KANG Li-hu,LUO Yu-ping,JIANG Ye-gen and MA Hai-bin.Medium Optimization of Acacia Rhizobia and Phosphobacteria and the Study of Phosphate-dissolving Ability[J].Forest Research,2005,18(2):177-182.
Authors:SHANG Jun-hong  KANG Li-hu  LUO Yu-ping  JIANG Ye-gen and MA Hai-bin
Institution:Research Institute of Tropical Forestry, CAF, Guangzhou510520, Guangdong, China;;Research Institute of Tropical Forestry, CAF, Guangzhou510520, Guangdong, China;Nanchang University, Nanchang330047, Jiangxi, China;Research Institute of Tropical Forestry, CAF, Guangzhou510520, Guangdong, China;Research Institute of Tropical Forestry, CAF, Guangzhou510520, Guangdong, China
Abstract:The growth curves of MZ rhizobium and 217 phosphobacteria were drawn in four media in this study. The results showed that these two strains could both grow well in BSE medium. Based on the conventional media, the optimized media of MZ rhizobia and 217 phosphobacteria were obtained from orthogonal tests. Furthermore, the optimized media were testified to be more suitable for the growth of both of the strains, with more live bacteria, by the growth curves got afterwards. The tight relationship between pH level, phosphate-dissolving and bacterial growth ability was also discussed in the study on the phosphate-dissolving ability.
Keywords:Acacia  rhizobia  phosphobacteria  media optimization  phosphate-dissolving ability
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