一株生防细菌的分离鉴定及其抑菌活性物质的分析

随着人们对农产品品质和安全性要求的提高，高效生物防治资源的筛选显得尤其重要。本研究通过平板对峙实验、分子鉴定、刚果红染色、葡聚糖酶基因克隆与序列分析等方法获得一株抑制多种植物病原真菌的芽孢杆菌，编号为YW26。16S rDNA基因序列分析表明该菌株为解淀粉芽孢杆菌；刚果红染色显示该菌株具有很强的产纤维素酶能力；根据GenBank数据库中芽孢杆菌内切葡聚糖酶基因(CP000560)序列设计引物，克隆得到1527 bp的片段，经测序、BLAST分析显示该片段与Bacillus amyloliquefaciens FZB42内切β-1,4-葡聚糖酶基因序列同源性为99%。该解淀粉芽孢杆菌抑菌效果显著，具有较强产纤维素酶能力。因此，该菌株可作为一种生防资源，开发新的生防制剂。

Isolation and Identification of an Bacillus amyloliquefaciens and study of its antibacterial activity

With the improvement of people’s requirements of the agricultural product quality and safety, efficient screening of biological control of resources becomes particularly important. A Bacillus, numbered YW26, inhibiting a variety of plant pathogenic fungi was isolated and identified by plate confrontation experiments, molecular identification, Congo red staining, cloning of glucanase gene and its sequence analysis in this study. 16S rDNA gene sequence analysis showed that it was a strain of Bacillus amyloliquefaciens. The strain had a strong cellulase production capacity by Congo red staining analysis. Primers were designed according to Bacillus endoglucanase gene (CP000560) in the GenBank database, a 1527 bp fragment was cloned, and the fragment had 99% homology with endo-β-1,4-glucanase gene sequence of Bacillus amyloliquefaciens FZB42 by sequencing and BLAST analysis. The Bacillus amyloliquefaciens strain had significant effect of inhibiting pathogens as well as strong cellulase production capacity. Therefore, the strain can be used as biological control resources to develop new biological control agents.