Detection of tobacco rattle virus in potato tubers using a simple RT-PCR procedure |
| |
| Authors: | Mogens Nicolaisen Zoltán Bösze Steen Lykke Nielsen |
| |
| Affiliation: | (1) Department of Plant Protection, Research Centre Flakkebjerg, Danish Institute of Agricultural Sciences, DK-4200 Slagelse, Denmark;(2) Plant Protection Institute, Pannon University of Agricultural Sciences, H8360 Keszthely, Hungary |
| |
| Abstract: | Summary A simple assay for detection of tobacco rattle virus in infected tubers involving RT-PCR was developed. The assay detected a wide range of strains, including nonparticle NM variants. RNA extraction methods were compared, and a simple method was found to give reliable results. The method enabled detection of at least 1 ng of tobacco rattle particles in 100 mg tuber extract. |
| |
| Keywords: | spraing potato mop-top virus |
| 本文献已被 SpringerLink 等数据库收录！ |
