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| 东北天南星体细胞胚胎发生及植株再生体系的优化 | |
| 引用本文： | 冯颖,张勋,张伟,信天朋,顾地周.东北天南星体细胞胚胎发生及植株再生体系的优化[J].贵州农业科学,2010,38(5). |
| 作者姓名： | 冯颖 张勋 张伟 信天朋 顾地周 |
| 作者单位： | 1. 通化师范学院,生物系,吉林,通化,134002 2. 武警通化市支队,卫生队,吉林,通化,134001 3. 通化市第十三中学分校,吉林,通化,134002 |
| 基金项目： | 国家科技攻关计划引导项目，通化师范学院自然科学基金 |
| 摘 要： | 为建立东北天南星体细胞胚胎发生体系,以东北天南星幼叶为外植体,应用均匀设计法筛选其最适合的胚性愈伤组织及胚性细胞复合体诱导、体细胞胚胎发育及植株再生的培养基。结果表明,东北天南星叶片胚性愈伤组织及胚性细胞复合体诱导最适宜的培养基为MS+6-BA 2.5 mg/L+2,4-D 2.0 mg/L+IAA 0.1 mg/L,诱导率为98%;体细胞胚胎发育及植株再生最适宜的培养基为MS+6-BA 0.9 mg/L+NAA 0.1 mg/L+IAA 0.1 mg/L,体细胞胚萌发率为99.5%,萌发的胚20 d后全部发育成完整植株。 |
| 关 键 词： | 东北天南星 均匀设计 胚性细胞复合体 体细胞胚 植株再生 |
Selection of Optimum Media for Somatic Embryogenesis and Plantlet Generation of Arisaema amurense |
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| FENG Ying,ZHANG Xun,ZHANG Wei,XIN Tian-peng,GU Di-zhou.Selection of Optimum Media for Somatic Embryogenesis and Plantlet Generation of Arisaema amurense[J].Guizhou Agricultural Sciences,2010,38(5). | |
| Authors: | FENG Ying ZHANG Xun ZHANG Wei XIN Tian-peng GU Di-zhou |
| Institution: | FENG Ying1,ZHANG Xun2,ZHANG Wei3,XIN Tian-peng1,GU Di-zhou1(1.Department of Biology,Tonghua Normal University,Tonghua,Jilin 134002,2.Medical Team,Tonghua Division of Armed Police,Jilin 134001,3.Branch School of Tonghua NO.13 Middle School,China) |
| Abstract: | Young leaves of Arisaema amurense were used as explants to screen out the optimum media for embryonic callus and embryonic cell complex induction,somatic embryo development and plantlet regeneration by using the uniform design to establish a somatic embryogenesis system of Arisaema amurense.The results showed that the optimum medium for 98% induction rate of embryonic callus and embryonic cell complex was MS+6-BA 2.5mg/L+ 2,4-D 2.0mg/L +IAA 0.1mg/L,and the optimum medium for 99.5% emergence rate of somatic ... |
| Keywords: | Arisaema amurense uniform design embryonic cell complex somatic embryo plantlet regeneration |
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