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南方红豆杉种子不同溶剂提取液对白菜种子萌发及幼苗生长的化感效应
引用本文:张艳杰,鲁顺保,高捍东.南方红豆杉种子不同溶剂提取液对白菜种子萌发及幼苗生长的化感效应[J].中国农学通报,2010,26(9):190-194.
作者姓名:张艳杰  鲁顺保  高捍东
作者单位:1. 江西师范大学生命科学学院,南昌,330022
2. 南京林业大学森林资源与环境学院,南京,210037
基金项目:江西师范大学博士启动基金
摘    要:通过生物检测方法研究了南方红豆杉种子的种皮和胚乳的石油醚、甲醇、乙酸乙酯、乙醚以及水浸提液对白菜籽发芽率和幼苗生长的化感作用,结果表明,其浸提液对白菜发芽及生长均有抑制作用,且随浸提液浓度增大抑制作用增强。种皮和胚乳甲醇相抑制物质对种子的萌发及生长抑制活性最强,抑制率达100%,种皮的石油醚相、乙醚相和乙酸乙酯相对发芽率的抑制作用强于胚乳,胚乳水相的抑制作用强于种皮;对白菜苗高生长的抑制作用,水相中低浓度处理的种皮抑制作用大于胚乳,高浓度处理时则相反;种皮石油醚相和乙酸乙酯相对白菜根长生长抑制作用大于胚乳,而乙醚相和水相中则相反。胚乳中各相对白菜苗高生长的抑制作用由强到弱依次甲醇相、乙酸乙酯相、乙醚相、石油醚相、水相;对发芽率和根长生长抑制作用由大到小依次甲醇相、乙醚相、乙酸乙酯相、水相和石油醚相。种皮中各相对苗高和根长生长的抑制作用由强到弱依次甲醇相、石油醚相、乙酸乙酯相、乙醚相和水相,对白菜籽发芽率的抑制作用由大到小依次为:甲醇相、乙醚相、乙酸乙酯相、石油醚相和水相。

关 键 词:中介蝮蛇    中介蝮蛇    纤溶酶    基因克隆    序列分析
收稿时间:2009-12-14
修稿时间:2009-12-21

Allelopathic Effect of Different Solvent Extraction from Seed of Taxus chinensis var.mairei on Cabbage Seed Germination and Seedling Growth
Zhang Yanjie,Lu Shunbao,Gao Handong.Allelopathic Effect of Different Solvent Extraction from Seed of Taxus chinensis var.mairei on Cabbage Seed Germination and Seedling Growth[J].Chinese Agricultural Science Bulletin,2010,26(9):190-194.
Authors:Zhang Yanjie  Lu Shunbao  Gao Handong
Institution:1College of Life Science Jiangxi Normal University, Nanchang 330022; 2College of Forest Resources and Environment Nanjing Forestry University, Naniing 210037
Abstract:In this paper, the allelopathy of the extracts of petroleum ether, methanol, ethyl acetate, aether and water from seed coat and endosperm of Taxus chinensis var. mairei was studied through bioassay method. The results showed that there were inhibitory effects in extracts on the germination and growth of cabbage, and the inhibitory effects became stronger as the contents of extracting solutions increased. Inhibitory of the extract of methanol from seed coat and endosperm on germination and growth was the strongest, and the inhibition rate was 100%. The inhibitory effects on the extracts of petroleum ether, aether and ethyl acetate were stronger than the endosperm in seed coat, while the inhibition of extracts of water of endosperm was stronger than the seed coat. To inhibit the growth of cabbage seedling height, the inhibition of extracts of water of seed coat was stronger than the endosperm in low concentrations, while high-density processing showed the opposite results. To inhibit the growth of cabbage root length, the inhibition of extracts of petroleum ether and ethyl acetate of seed coat were stronger than the endosperm, while the inhibition of extracts of aether and water showed the opposite results. The inhibitory effects of endosperm extractions on the growth of cabbage seedling height couldbe listed as, from strongest to weakest, the extraction phase of methanol, ethyl acetate, aether, petroleum ether and water; while the effects on the growth of cabbage seedling root and germination percentage could be listed as, methanol, aether, ethyl acetate, water and petroleum ether. The inhibitory effects of seed coat extraction solutions to the growth of cabbage seedling height and root could be listed as, of methanol, of petroleum ether, of ethyl acetate, of aether and of water, while the effects on the growth of cabbage seedling germination percentage could be listed as, methanol, aether, ethyl acetate, petroleum ether and water.
Keywords:Taxus chinensis var  mairei  seed  extract  allelopathy
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