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| 生防木霉菌对嘧霉胺抗药性变异及其相关蛋白诱导 | |
| 引用本文： | 陈红漫,陈捷,阚国仕,李贺民. 生防木霉菌对嘧霉胺抗药性变异及其相关蛋白诱导[J]. 植物保护学报, 2005, 32(1): 77-80 |
| 作者姓名： | 陈红漫 陈捷 阚国仕 李贺民 |
| 作者单位： | 1. 沈阳农业大学生物科技学院,沈阳,110161 2. 上海交通大学农业与生物学院,上海,201101 |
| 摘 要： | 选育对杀菌剂具有抗性的生防菌株是生物防治研究的重要内容之一.通过药剂选择培养基及紫外光诱导获得对嘧霉胺具抗性的突变体KTm-1、KTm-2.研究结果显示,与亲本菌株相比,抗性突变体的耐药性提高20倍以上.抗性菌株在无药培养基上连续转代8次,耐药性未见下降.活体拮抗测定表明,耐药菌株的抑菌能力略高于亲本菌株.PAGE显示抗性突变体KTm-1的可溶性蛋白谱带比其亲本菌株多4条,其中Rf=0.64的蛋白谱带是两个耐药菌株的特异性谱带,可视其为耐药相关蛋白.经Sephadex G-100柱层析纯化,SDS-PAGE测定耐药蛋白分子量为44.1KDa. |
| 关 键 词： | 木霉 嘧霉胺 耐药性 突变体 |
| 收稿时间： | 2003-10-17 |
| 修稿时间： | 2003-10-17 |
Mutation of Trichoderma in tolerance to pyrimethanil and induction of related protein |
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| CHEN Hong-man,CHEN Jie,KAN Guo-shi and LI He-min. Mutation of Trichoderma in tolerance to pyrimethanil and induction of related protein[J]. Acta Phytophylacica Sinica, 2005, 32(1): 77-80 | |
| Authors: | CHEN Hong-man CHEN Jie KAN Guo-shi LI He-min |
| Affiliation: | College of Biotechnology, Shenyang Agricultural University, Shenyang 110161, China;College of Agricultural and Biology, Shanghai Communication University, Shanghai 201101, China;College of Biotechnology, Shenyang Agricultural University, Shenyang 110161, China;College of Biotechnology, Shenyang Agricultural University, Shenyang 110161, China |
| Abstract: | Induce of biocontrol strain tolerance of Trichoderrna to chemical fungicides was studied.Two mu- tants tolerant to pyrimethanil were obtained by UV-light induction on PDA amended with pyrimethaniol from a wild Trichoderma strain.Tolerance level was over 20 times in comparison with that of the mother strain.The mutants could maintain their tolerance after 8 times of transfers on the fungicide free PDA.The soluble protein profile bands of tolerant strains were higher than that of their original sensitive strains among which,the toler- ant strains owned the soluble protein profile band of R_f=0.64.But it was not shown in sensitive strains,and it is called related tolerant protein.This protein was purified from KTm-1 by Sephadex G-100 gel filtration. The molecular weight of tolerant protein was determined to be 44.1 KDa.Two tolerant strains kept the ability of antagonism against Botrytis cinerea in vitro and vivo. |
| Keywords: | Trichoderma pyrimethaniol tolerance mutant |
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