Quantitative capillary reversed passive latex agglutination test for C-reactive protein (CRP) in the dog |
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Authors: | K Tagata S Yokoyama T Ginbo M Honda T Okimura M Odakura M Nomura S Yamamoto |
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Institution: | (1) Tagata Animal Hospital, 2-chome 23-14 Koshibara, 690 Matsue, Shimane, Japan;(2) Department of Immunology, College of Environmental Health Science, Azabu University, 229 Sagamihara, Kanagawa, Japan;(3) Department of Hygiene, College of Environmental Health Science, Azabu University, 229 Sagamihara, Kanagawa, Japan |
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Abstract: | A capillary reversed passive latex agglutination test (capillary RPLA) was developed which allows quantification of serum C-reactive protein (CRP) within approximately 15 min. The logarithmic regression line (calibration curve) obtained after measuring each CRP concentration three times in twofold dilutions of a standard canine serum containing 222 g/ml of CRP was y=6.394+0.030x (r=0.995). Capillary RPLA permitted quantification of CRP in the range 6.9–222 g/ml. The coefficients of variation ranged from 10.28% to 12.40%. The recovery rates (percentage recovery) of CRP by capillary RPLA were within the range 87% to 106%. On measuring the CRP concentrations in sera from 78 dogs by capillary RPLA, single radial immunodiffusion (SRID) and enzyme-linked immunosorbent assay (ELISA), close correlations were demonstrated between SRID and capillary RPLA (y=7.250+1.109x, r=0.978), between SRID and ELISA (y=3.042+1.059x, r=0.967), and between capillary RPLA and ELISA (y=1.778+0.929x, r=0.962). Capillary RPLA may be considered useful as a routine biochemical technique for measurement of serum CRP concentration in the dog.Abbreviations CRP
C-reactive protein
- ELISA
enzyme-linked immunosorbent assay
- RPLA
reversed passive latex agglutination test
- SRID
single radial immunodiffusion |
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Keywords: | agglutination capillary RPLA C-reactive protein ELISA latex single radial immunodiffusion |
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