异养小球藻细胞采收方法的研究

异养小球藻体积小、发酵液成分复杂，导致藻细胞采收困难、采收成本高。通过比较三氯化铁、硫酸铝、壳聚糖、聚丙烯酰胺、霉菌5种絮凝剂，并利用单因素试验、响应面分析对三氯化铁絮凝法收集异养小球藻进行工艺条件优化，获得了二次回归模型。得到了最优变量为三氯化铁添加量2.0 g·L^-1、搅拌速率150 r·min^-1、搅拌时间20 min，在此条件下，异养小球藻采收率达到95.3%，收集成本为0.07元·kg^-1。采收率高、采收成本低，为工业化应用奠定了基础。

Study on the Collecting Method of Heterotrophic Chlorella Cells

The small volume of heterotrophic Chlorella and the complex components of fermentation broth make it difficult in algae cell harvesting and high recovery cost. In this study, five flocculant of ferric chloride, aluminum sulfate, chitosan, polyacrylamide and mold were compared, single factor test and response surface analysis were used to optimize the process conditions of collecting heterotrophic Chlorella by ferric chloride flocculation method, and a quadratic regression model was obtained. The optimal variables were the dosage of ferric chloride 2.0 g·L^-1, agitation rate 150 r·min^-1, agitation time 20 min. Under this condition, the recovery rate reached 95.3%, and the collection cost was 0.07 yuan·kg^-1. The recovery rate was high and the recovery cost was low, which laid the foundation for the industrialization application.