东方百合‘索邦’的离体培养研究

东方百合‘索邦’作为市售主流鲜切花百合品种，具有巨大的市场潜力。为满足市场需求，利用花器官作为初始材料，对东方百合‘索邦’离体培养涉及的愈伤组织诱导、丛生芽增殖及生根培养等相关技术进行研究。结果表明，子房为合适的初始诱导外植体，进一步扩繁诱导愈伤组织时适宜选用叶片下部或鳞茎片为外植体，合适的愈伤组织诱导培养基为MS+2.0 mg·L^-1 2,4-D+0.2 mg·L^-1 6-BA +30 g·L^-1 蔗糖+6.5 g·L^-1 琼脂，pH 5.8。丛生芽增殖培养基为MS+1.0 mg·L^-1 6-BA+0.1 mg·L^-1 NAA +30 g·L^-1 蔗糖+6.5 g·L^-1 琼脂，pH 5.8。生根培养基为MS+0.5 mg·L^-1 NAA +30 g·L^-1 蔗糖+6.5 g·L^-1 琼脂，pH 5.8。结果为进一步完善东方百合‘索邦’的离体培养体系，进行优质种球的规模化生产提供基础。

Study on in vitro Cultivation of Lilium orential ‘Sorbonne’

As the main market fresh cut lily variety, Lilium orential ‘Sorbonne’ has great market potential. In order to meet the market demand, flower organs were used as initial materials to study the callus induction, cluster bud proliferation and rooting culture of L. orential ‘Sorbonne’ in vitro culture. The results showed that ovary was the appropriate initial explant for callus induction, and the lower part of leaf or bulb was the appropriate explant for further propagation. The suitable medium for callus induction was MS+ 2.0 mg·L^-1 2,4-D+0.2 mg·L^-1 6-BA+30 g·L^-1 sucrose+6.5 g·L^-1 agar, pH 5.8. Cluster bud proliferation medium was MS+ l.0 mg·L^-1 6-BA + 0.l mg·L^-1 NAA +30 g·L^-1 sucrose+6.5 g·L^-1 agar, pH 5.8. Rooting culture medium was MS+0.5 mg·L^-1 NAA+30 g·L^-1 sucrose+6.5 g·L^-1 agar, pH 5.8. The results provided a basis for further improving the in vitro culture system of L. orential ‘Sorbonne’ and for large-scale production of high-quality seed bulbs.