| 东北虎巴氏杆菌病的病原分离鉴定及耐药性分析 |
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| 引用本文： | 宋德光,贺文琦,陆慧君,佟庆彬,成军,高丰.东北虎巴氏杆菌病的病原分离鉴定及耐药性分析[J].中国预防兽医学报,2007,29(1):13-16. |
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| 作者姓名： | 宋德光 贺文琦 陆慧君 佟庆彬 成军 高丰 |
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| 作者单位： | 1. 吉林大学,畜牧兽医学院,吉林,长春,130062
2. 吉林市江南公园,吉林,吉林,132013 |
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| 摘 要： | 为确定从死亡东北虎脏器组织中分离获得的1株细菌的特性，通过菌体形态、菌落形态、革兰氏和美蓝染色特性、生化特性、血清型、动物致病性试验等系统鉴定，确定其为高致病性的多杀性巴氏杆菌。20种常用抗菌药物的纸片法药敏试验结果证明，该菌株仅对新霉素、头孢唑啉和卡那霉素3种药物敏感，对大多数抗菌药物高度耐药。经电镜负染观察及传代细胞系和鸡胚进行病毒分离，结果均为阴性。
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| 关 键 词： | 东北虎 巴氏杆菌 分离鉴定 耐药性 |
| 文章编号： | 1008-0589（2007）01-0013-04 |
| 收稿时间： | 2006-04-06 |
| 修稿时间： | 2006年4月6日 |
Isolation, identification and resistance of pasteurellosis pathogeny in a Siberian tiger |
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| SONG De-guang,HE Wen-qi,LU Hui-jun,TONG Qing-bin,CHENG Jun,GAO Feng.Isolation, identification and resistance of pasteurellosis pathogeny in a Siberian tiger[J].Chinese Journal of Preventive Veterinary Medicine,2007,29(1):13-16. |
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| Authors: | SONG De-guang HE Wen-qi LU Hui-jun TONG Qing-bin CHENG Jun GAO Feng |
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| Abstract: | A bacterium isolated from a tiger was identified as serotype Fg and capsule type A, pasteurella multocida by morphological, germiculture, staining, biochemical characteristics, and capsular tests. The animal regression test showed that the bacterium has high pathogenicity. The result of resistance test to 20 drugs showed that the bacterium was sensitive to neomycin, cefazolin and kanamycin, but highly resistant to another drugs that were used. Virus particle was not detected through negative staining by electron microscope and virus isolated according to the cells and chicken embryos. |
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| Keywords: | Siberian tiger pasteurella isolation and identification resistance |
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