猪微卫星多态性两种检测方法的比较研究

 为了确定一种安全、有效的检测猪微卫星多态性的实验方法,采用两种电泳和两种染色方法对大白猪基因组DNA进行了6个微卫星座位的PCR扩增,并将扩增产物在2.5%的琼脂糖凝胶上电泳,EB染色,然后又在8%的聚丙烯酰胺凝胶上电泳,AgNO₃染色。结果表明两种方法检测出的等位基因数目和基因多态性水平存在差异,8%的聚丙烯酰胺凝胶电泳在区分微小差异片段能力上明显优于2.5%的琼脂糖凝胶电泳。

Study on Comparison of Two Methods for Detecting Microsatellite Polymorphism in Pig

For the purpose of founding a kind of safe and efficient method to reveal the microsatellite polymorphism of pig,two methods(2.5% agarose gel electrophoresis and 8% polyacrylamide gel electrophoresis)were applied to detect polymorphism of 6 microsatellite loci in Large White pig.Result showed there was difference from the two methods that detect the number of allele and gene polymorphism,resolution of 8% polyacrylamide gel electrophoresis was higher than that of 2.5% agarose gel electrophoresis,the former has stronger ability of distinguishing between microscopical discrepancy fragments.