6-BA浸种对HgCl2胁迫下小麦种子萌发和幼苗生长的影响

为探讨6-苄基腺嘌呤（6-BA）浸种对HgCl₂胁迫下小麦种子萌发及其幼苗生长的缓解作用，以3个小麦品种为材料，研究了不同浓度6-BA浸种对200 mg·L^-1HgCl₂胁迫下小麦种子发芽、幼苗生长、抗氧化酶活性、可溶性蛋白和丙二醛含量的影响。结果表明，200 mg·L^-1HgCl₂处理后，3个小麦品种种子的发芽势、发芽率、根长、芽长和鲜重降低，幼苗的抗氧化酶活性和可溶性蛋白含量降低，丙二醛含量增加。一定浓度6-BA浸种可以缓解HgCl₂胁迫对小麦种子萌发的抑制作用，但对小麦的根长、芽长和鲜重的影响因小麦品种而不同。HgCl₂胁迫下，3个小麦品种幼苗的SOD、POD活性和可溶性蛋白含量随着6-BA浓度的增加均基本呈先升高后降低的趋势，最佳6-BA浓度因品种而不同；3个小麦品种的MDA含量均呈先降低后升高的趋势，以6-BA浓度为15 mg·L^-1时MDA含量最低。说明一定浓度6-BA浸种能够缓解HgCl₂胁迫对小麦种子萌发和幼苗生长的毒害作用，但最佳6-BA浓度因小麦品种的不同而不同。

Effect of 6-BA Seed Soaking on Seed Germination and Seedling Growth of Wheat under Mercuric Chloride Stress

To study the mitigation effect of 6-Benzylaminopurine(6-BA) seed soaking on seed germination and seedling growth of wheat under mercury chloride stress,three wheat cultivars were conducted in this experiment to study the effect of 6-BA seed soaking on seed germination,seedling growth,antioxidant enzyme activities,and the content of soluble protein and MDA.The results showed that treated wheat seed with 200 mg·L^-1 HgCl₂,the germination energy,germination rate,root length,bud length,and fresh weight of three varieties declined,and the antioxidant enzyme activities,the content of soluble protein of seedlings declined,but the MDA content increased.A certain concentration of 6-BA seed soaking could mitigate the inhibited effect of seed germination under mercury chloride stress,which promoted the germination of three wheat varieties,but the root length,bud length and fresh weight changed differently due to different varieties.Under HgCl₂ stress,the SOD,POD activities and soluble protein content of three wheat variety seedlings increased first and then decreased with the increasing of the 6-BA concentration.The most proper 6-BA concentration differed with different wheat varieties.The MDA content of three wheat varieties declined first and then increased,reaching the minimum under the concentration of 6-BA at 15 mg·L^-1.The results indicated that certain concentration of 6-BA seed soaking could mitigate the toxicity effect of HgCl₂ stress on seed germination and seedling growth of wheat,but the most proper 6-BA concentration was different among wheat varieties.