内蒙古野生华北驼绒藜种群遗传多样性和遗传分化研究

本研究利用ISSR分子标记技术对内蒙古6个群落野生华北驼绒藜种群的遗传多样性和遗传变异进行了分析,结果表明,内蒙古不同种群野生华北驼绒藜种水平遗传多样性较高,多态位点百分率(PPB)达到98.05%,Nei’s基因多样性指数(h)和Shannon多样性指数(I)分别为0.298 4和0.455 7;依据PPB、h和I估计的各种群内平均遗传多样性的变化趋势一致,依次为PA＞PB＞PD＞PC＞PE＞PF。华北驼绒藜遗传变异主要存在于种群内。种群间的基因流(Nm)为4.333 2,大于1。根据聚类分析,大致可以将6个群落华北驼绒藜种群分为荒漠化草原种群和典型草原种群。生境片断化目前尚不足以导致华北驼绒藜特定基因丢失,发生遗传漂变的可能性很小。

Genetic diversity and genetic variation of Ceratoides arborescens wild populations in Inner Mongolia

By using inter-simple sequence repeat (ISSR) markers technique, this paper studied the genetic diversity and variation of six wild populations of Ceratoides arborescens. The results showed that at species level the percentage of polymorphic bank (PPB) was 98.05%, Nei’s gene diversity (h) and Shannon’s index (I) were 0.298 4 and 0.455 7, respectively. According to PPB, h and I, estimated the trend line of the average genetic diversity within each population was similar, the order was PA>PB>PD>PC>PE>PF. There were more genetic variations within the population than among populations. The gene flow is 4.333 2 among populations, were greater than 1. The cluster analysis suggested that the six populations of C. arborescens could be divided into two groups, i.e. desert grassland group and typical grassland group. The habitat fragmentation was still not enough to cause the loss of specific gene, genetic drift occured very unlikely.