Characterization of Verticillium dahliae Isolates from Potato on Hokkaido by Random Amplified Polymorphic DNA (RAPD) and REP-PCR Analyses |
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| Authors: | Tsutomu KOMATSU Akio SUMINO Koji KAGEYAMA |
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| Institution: | (1) Hokkaido Ornamental Plants and Vegetables Research Center, Takikawa 073–0026, Japan, JP;(2) Hokkaido Central Agricultural Experiment Station, Naganuma, Yubari 069–1395, Japan, JP;(3) Faculty of Agriculture, Gifu University, Gifu 051–1193, Japan, JP |
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| Abstract: | Verticillium dahliae isolates from potato on the island of Hokkaido (potato isolates) and those belonging to pathotypes A (eggplant pathotype),
B (tomato pathotype) and C (sweet pepper pathotype) were divided into three distinct groups by RAPD and REP-PCR. The three
DNA groups I, II, III consisted of pathotypes A and C, pathotype B and potato isolates, respectively. The potato isolates
were assigned to pathotype A on the basis of pathogenicity. Another set of potato isolates was further collected from eight
potato cropping regions on Hokkaido to further examine the relationships among them in detail. Only one of these isolates
was identified as DNA group II, but all the others were classified as DNA group III. Isolates from daikon, eggplant, and melon
on Hokkaido also belonged to DNA group III. These results suggest that V. dahliae isolates from Hokkaido are unique at the DNA level and different from other pathotype A isolates in Japan.
Received 28 February 2000/ Accepted in revised form 6 November 2000 |
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| Keywords: | : Verticillium dahliae potato isolate pathotype DNA group REP-PCR RAPD |
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