|
|||||
|
|
| 牡丹组织培养若干影响因子研究 | |
| 引用本文： | 殷丽青,王新其,胡永红,刘炤,李秀芬,张建军. 牡丹组织培养若干影响因子研究[J]. 上海农业学报, 2012, 28(1): 17-21 |
| 作者姓名： | 殷丽青 王新其 胡永红 刘炤 李秀芬 张建军 |
| 作者单位： | 1. 上海市农业科学院林木果树研究所,上海201403;上海城市植物资源开发应用工程技术研究中心,上海200231 2. 上海市农业科学院林木果树研究所,上海,201403 3. 上海城市植物资源开发应用工程技术研究中心,上海200231;上海植物园,上海200231 |
| 基金项目： | 上海市科技兴农重点攻关项目(沪农科攻字2008第10-4号);上海市重点科技攻关项目(10391901200,10dz2253700)资助 |
| 摘 要： | 以‘太平红’牡丹为材料,研究了不同灭菌方式、抗褐化物质、激素配比、培养基、生根培养条件对牡丹组织培养的影响。结果表明:以0.1%升汞液消毒8 min,再用7%次氯酸钠溶液消毒8 min灭菌效果最佳,鳞芽、土芽和种子的污染率分别为0、4.5%和22.0%;在改良MS培养基中联合添加PVPP 0.5 g/L和AC 0.5 g/L可有效减少牡丹外植体的褐化;改良MS+BA 1.0 mg/L+NAA 0.2 mg/L+GA 0.3 mg/L+CH 0.5 g/L+PVPP0.5 g/L+AC 0.5 g/L适宜牡丹鳞芽的诱导;MS+BA 1.5 mg/L+NAA 0.2 mg/L+PVPP 1.0g/L适宜于牡丹芽的增殖;1/2MS+IBA 5 mg/L+AC 0.5 g/L适用于牡丹试管苗生根。 |
| 关 键 词： | 牡丹 组织培养 增殖 褐化 生根 |
Several factors influencing tissue culture of Paeonia suffruticosa |
|
| YIN LI-qing , WANG Xin-qi , HU Yong-hong , LIU Zhao , LI Xiu-fen , ZHANG Jian-jun. Several factors influencing tissue culture of Paeonia suffruticosa[J]. Acta Agriculturae Shanghai, 2012, 28(1): 17-21 | |
| Authors: | YIN LI-qing WANG Xin-qi HU Yong-hong LIU Zhao LI Xiu-fen ZHANG Jian-jun |
| Affiliation: | 1 (1 Forestry and Pomology Research Institute,SAAS,Shanghai 201403,China;2 Shanghai Botanical Garden,Shanghai 200231,China;3 Shanghai Engineering Research Center of Urban Plant Resource Development,Shanghai 200231,China) |
| Abstract: | The peony cultivar ’Taipinghong’ was used as a test material,and tissue culture of peony was studied under using different sterilization methods,anti-browning materials,hormone recipes, media and rooting conditions.The result showed that the effect of sterilizing with 0.1%mercuric chloride solution for 8 min and then with 7%sodium hypochlorite solution for 8 min was the best,in which the contamination rate was zero for the scaly buds,4.5%for the underground buds and 22.0% for the seeds;Adding 0.5 g/L PVPP and 0.5 g/L AC in modified MS medium could effectively mitigate browning of the peony explants;Modified MS + 1.0 mg/L BA + 0.2 mg/L NAA + 0.3 mg/L GA + 0.5 g/L CH + 0.5 g/L PVPP+0.5 g/L AC was suitable for the scaly bud induction;MS + 1.5 mg/L BA+0.2 mg/L NAA+ 1.0 g/L PVPP was suitable for the peony bud propagation;And 1/2 MS+ 5.0 mg/L IBA+ 0.5 g/L AC was suitable for the test-tube seedling rooting. |
| Keywords: | Paeonia suffruticosa Tissue culture Propagation Browning Rooting |
| 本文献已被 CNKI 万方数据 等数据库收录！ | |