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| 猪流行性腹泻病毒纤突蛋白基因克隆与序列分析 | |
| 引用本文： | 李建强,柳纪省,兰喜,程杰,杨敏,吴润,殷相平,李宝玉,李学瑞,杨彬.猪流行性腹泻病毒纤突蛋白基因克隆与序列分析[J].农业生物技术学报,2007,15(4):562-566. |
| 作者姓名： | 李建强 柳纪省 兰喜 程杰 杨敏 吴润 殷相平 李宝玉 李学瑞 杨彬 |
| 作者单位： | 1. 甘肃农业大学动物医学院,兰州,730070;中国农业科学院兰州兽医研究所,家畜疫病病原生物学国家重点实验室,农业部畜禽病毒学重点开放实验室,兰州,730046 2. 中国农业科学院兰州兽医研究所,家畜疫病病原生物学国家重点实验室,农业部畜禽病毒学重点开放实验室,兰州,730046 3. 甘肃农业大学动物医学院,兰州,730070 |
| 基金项目： | 国家重点基础研究发展计划(973计划) |
| 摘 要： | 对猪流行性腹泻病毒(Porcine epidemic diarrhea virus,PEDV)DX株纤突蛋白基因进行了克隆与测序。结果表明,该基因核苷酸序列长为4152bp,推导氨基酸序列为1383aa,与比较毒株氨基酸同源性都在90%以上。系统发生树分析结果表明,比较毒株可分为3个基因群,而且每个群的毒株均来自同一个国家。利用生物软件分析结果表明PEDV发生微小的变异,尤其在韩国变异明显,而DX株与中国JS-2004-2株亲缘关系很近。 |
| 关 键 词： | 猪源冠状病毒 猪流行性腹泻病毒 纤突蛋白基因 克隆 特征分析 |
| 文章编号： | 1006-1304（2007）04-0562-05 |
| 收稿时间： | 2006-12-18 |
| 修稿时间： | 2007-02-08 |
Cloning and Characterization Analysis of Spike Protein Gene of Porcine epidemic diarrhea virus |
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| LI Jian-qiang,LIU Ji-xing,LAN Xi,CHENG Jie,YANG Min,WU Run,YING Xiang-ping,LI Bao-yu,LI Xue-rui,YANG Bin.Cloning and Characterization Analysis of Spike Protein Gene of Porcine epidemic diarrhea virus[J].Journal of Agricultural Biotechnology,2007,15(4):562-566. | |
| Authors: | LI Jian-qiang LIU Ji-xing LAN Xi CHENG Jie YANG Min WU Run YING Xiang-ping LI Bao-yu LI Xue-rui YANG Bin |
| Institution: | 1. College of Vetennary Medicine, Gansu Agricultural University ,Lanzhou 730070,China; 2. Key Laboratory of Animal Virology, Ministry of Agriculture, State Key Laboratory of Veterinary Etiological Biology, Lanzhou Vetednary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou 730046,China |
| Abstract: | The spike protein gene of Porcine epidemic diarrhea virus(PEDV)DX strain was cloned and sequenced.The results showed that the nucleotide sequence of the gene was 4 152 bp in length and encoding a protein of 1 383 aa,the homology of amino acids between it and other PEDV strains exceeded 90%.The results of phylogenetic tree analysis showed that all the reference PEDV isolates could be divided into 3 genetic groups and each of them in the same group came from the same country.Bio-soft analysis showed that PEDV mutated slowly,especially in Korea.And DX strain and JS-2004-2 strain in China had a closed relationship. |
| Keywords: | Porcine epidemic diarrhea virus spike gene cloning characterization analysis |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ | |