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| 微卫星标记在近交系及封闭群小鼠遗传质量检测中的初步应用 | |
| 引用本文： | 谢建云,冯洁,胡建华,周艳,邵伟娟,倪丽菊,魏晓锋,高诚. 微卫星标记在近交系及封闭群小鼠遗传质量检测中的初步应用[J]. 上海交通大学学报(农业科学版), 2008, 26(4) |
| 作者姓名： | 谢建云 冯洁 胡建华 周艳 邵伟娟 倪丽菊 魏晓锋 高诚 |
| 作者单位： | 上海实验动物研究中心,上海市实验动物监督检验站,上海,300032;上海计划生育科学研究所,上海,200032;上海实验动物研究中心,上海市实验动物监督检验站,上海,300032;杨州大学,比较医学中心,杨州,225009 |
| 摘 要： | 利用建立的小鼠微卫星检测方法,对来源于2个单位的近交系BALB/c小鼠和封闭群ICR小鼠进行了遗传分析,结果发现A单位BALB/c小鼠发生了遗传污染,有部分小鼠在部分微卫星位点发生了变异,而B单位BALB/c小鼠在所有的检测位点上的结果都与预期的BALB/c小鼠位点一致;对ICR 小鼠分析结果发现A单位ICR小鼠在22个位点的平均杂合度为0.379,平均多态信息量为0.308,属中度多态,B单位ICR小鼠的平均杂合度为0.287,平均多态信息量为0.227,属低度多态.结果表明微卫星标记可用于检测近交系小鼠的遗传质量,也可用于分析封闭群小鼠的遗传多态性,为一种有潜力的实验动物遗传学检测标记. |
| 关 键 词： | 微卫星标记 近交系小鼠 封闭群小鼠 遗传检测 遗传多态性 |
Application of Microsatellite Marker in Genetic Quality Detection of Inbred and Closed Colony |
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| XIE Jian-yun FENG Jie HU Jian-hua ZHOU Yan SHAO Wei-juan NI Li-ju WEI Xiao-feng GAO Cheng angzhou University,Yangzhou,China. Application of Microsatellite Marker in Genetic Quality Detection of Inbred and Closed Colony[J]. Journal of Shanghai Jiaotong University (Agricultural Science), 2008, 26(4) | |
| Authors: | XIE Jian-yun FENG Jie HU Jian-hua ZHOU Yan SHAO Wei-juan NI Li-ju WEI Xiao-feng GAO Cheng angzhou University Yangzhou China |
| Affiliation: | XIE Jian-yun~(1,2) FENG Jie~1 HU Jian-hua~1 ZHOU Yan~1 SHAO Wei-juan~1 NI Li-ju~1 WEI Xiao-feng~1 GAO Cheng~1 (1.Shanghai Quality Monitoring Center for Laboratory Animals,Shanghai Laboratory Animal Research Center,Shanghai 200032,China,2.Shanghai Institute of Planned Parenthood Research,3.Comparodive Medicine Center,(?)angzhou University,Yangzhou 225009,China) |
| Abstract: | To analyze the genetic quality of BALB/c and ICR mice from two units.The result showed that the BALB/c mice from Unit A was genetic contaminated,some of the mice were mutated in some microsatellite loci,and BALB/c mice from Unit B was coinci- dence with the expecting result of the standard BALB/c strain.The ayerage heterozygosity and polymorphism information content of 22 microsatellite loci of ICR mice from Unit A was 0.379 and 0.308 respectively,this indicated that ICR mice from Unit A was medium genetic ... |
| Keywords: | microsateUite marker inbred mice closed colony genetic detection genetic polymorphism |
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