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| 烟草AFLP银染分析体系的建立 | |
| 引用本文： | 杜传印,王玉军,李斯深,李常保,刘洪祥,田纪春.烟草AFLP银染分析体系的建立[J].山东农业科学,2007(5):38-41,44. |
| 作者姓名： | 杜传印 王玉军 李斯深 李常保 刘洪祥 田纪春 |
| 作者单位： | 1. 山东潍坊烟草有限公司,山东,潍坊,261031;山东农业大学农学院,山东,泰安,271018;中国烟草总公司青州烟草研究所,山东,青岛,266061 2. 山东农业大学植保学院,山东,泰安,271018 3. 山东农业大学农学院,山东,泰安,271018 4. 中国烟草总公司青州烟草研究所,山东,青岛,266061 |
| 摘 要： | 以14个烟草品种为试材,初步建立了适合于烟草AFLP分析的银染分析体系:烟草基因组的DNA提取采用CTAB法;地酶切连接同步进行;预扩增选用E+A和M+C引物;选择性扩增选用E+3和M+3引物;扩增产物利用6%聚丙烯酰胺凝胶电泳,后用银染法检测。采用优化的体系,20对引物组合扩增出清晰的烟草AFLP图谱。 |
| 关 键 词： | 烟草 AFLP分析 银染体系 |
| 文章编号： | 1001-4942（2007）05-0038-05 |
| 修稿时间： | 2007-04-122007-04-24 |
Construction of AFLP Silver-staining Analysis System in Tobacco |
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| DU Chuan-yin,WANG Yu-jun,LI Si-shen,LI Chang-bao,LIU Hong-xiang,TIAN Ji-chun.Construction of AFLP Silver-staining Analysis System in Tobacco[J].Shandong Agricultural Sciences,2007(5):38-41,44. | |
| Authors: | DU Chuan-yin WANG Yu-jun LI Si-shen LI Chang-bao LIU Hong-xiang TIAN Ji-chun |
| Institution: | 1. Weif ang Tobacco Limited Corporation, Weif ang 261031 ,China; 2. Plant Protection College, Shandong Agricultural University, Taian 271018, China ; 3. Agronomy College, Shandong Agricultural University, Taian 271018 ,China ; 4. Qingzhou Tobacco Research Institute of CNTC, Qingdao 266061,China |
| Abstract: | AFLP(Amplified Fragment Length Polymorphism) silver-staining analysis system was established with 14 tobacco cultivars.The results of several reactions in the analysis system were as following: tobacco genomic DNA was extracted by CTAB method;the double enzymes restriction and the adapter-ligation were completed at the same time;E A and M C primer combinations were used in pre-amplification while E 3 and M 3 primer combinations in selective amplification;the PCR reaction products were analyzed on 6% denatured polyacrylamide gel and then stained by silver.With the optimized system,the AFLP fingerprinting of 3 tobacco cultivars amplified by 20 primer combinations had been obtained. |
| Keywords: | Tobacco AFLP Silver-staining analysis system |
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