肉鸡腹水综合征患鸡右心组织钙含量的研究

利用原子吸收分光光度法和焦锑酸钾组化电镜技术,测定肉鸡右心室壁总钙和右心室组织细胞内游离Ca2+含量和分布。结果显示肉鸡腹水综合征患鸡右心室壁总钙含量为(71.2±6.5)mg/kg,显著高于低温未发病和健康肉鸡(分别为58.0±4.4,46.8±3.1,P<0.01)。电镜观察发现Ca2+沉淀颗粒主要散在分布于患鸡心肌胞浆、肌浆网、线粒体等部位,腹水综合征患鸡Ca2+颗粒的沉积量比健康肉鸡显著增多。结果提示心肌细胞内Ca2+的异常可能与肉鸡腹水综合征的发生发展有关。

The Change of Calcium in Right Ventricle of Ascites Syndrome Broiler

The total calcium and Ca~(2+)in right ventricle of broiler were evaluated to study the relationship between calcium signal transduction and ascites syndrome (AS) in broiler chickens.The total calcium in right ventricle(RV) of broiler was measured by atomic absorption spectrophotometer.The result showed that the total calcium of RV with AS is significantly higher than that of broilers reared in low ambient temperature and normal temperature(P<0.01).The quantity and distribution of Ca~(2+) in right ventricle was observed by pyroantimonate precipitation.The result showed that Ca~(2+) was mainly located in cytoplasm,sarcoplasmic reticulum and mitochondrion.The quantity of Ca~(2+) in myocardium of broiler with AS was more than that of normal broiler.The increase of total calcium of RV and the precipitation of Ca~(2+) in the cardiac muscle cell of broiler with AS suggested that calcium abnormal play an important role in the development of AS.