Immune characterization of long pentraxin 3 in pigs infected with influenza virus |
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| Authors: | Elisa Crisci Lorenzo Fraile Sonia Valentino Laura Martínez-Guinó Barbara Bottazzi Alberto Mantovani Maria Montoya |
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| Institution: | 1. Centre de Recerca en Sanitat Animal (CReSA), UAB-IRTA, Campus de la Universitat Autònoma de Barcelona, 08193 Bellaterra, Barcelona, Spain;2. Universitat de Lleida, Lleida, Spain;3. Humanitas Clinical and Research Center, Rozzano, Italy;4. Department of Translational Medicine, University of Milan, Rozzano, Italy;5. Institut de Recerca i Tecnologia Agroalimentàries (IRTA), Barcelona, Spain |
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| Abstract: | Long pentraxin 3 (PTX3) is a conserved pattern-recognition secreted protein and a host-defence-related component of the humoral innate immune system. The aim of the present study was to characterize swine PTX3 (SwPTX3) protein expression in influenza virus infected pigs. First, we performed in silico studies to evaluate the cross-reactivity of PTX3 human antibodies against SwPTX3. Secondly, we used in vitro analysis to detect SwPTX3 presence in swine bone marrow dendritic cells (SwBMDC) upon stimulation with different agents by Western blot and immunofluorescence. Finally, the levels of SwPTX3 were assessed in experimental infection of pigs with different strains of influenza virus. This is a novel study where the expression of SwPTX3 was evaluated in the context of a pathogen infection. The initial characterization of SwPTX3 in influenza virus infected pigs contributes to understand the role of PTX proteins in the immune response. |
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| Keywords: | PTX3 Pigs DC Influenza virus |
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